AI Article Synopsis
- Researchers introduced a new oblique plane microscope (OPM) that improves image resolution and usability with a unique glass-tipped objective lens.
- This microscope offers impressive imaging capabilities, matching high-quality outputs similar to advanced lattice light-sheet microscopy but in a more user-friendly design.
- It successfully captures complex biological processes like endocytosis and cell migration in tight spaces, enabling studies that are difficult with traditional microscopy techniques.
Article Abstract
We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing to its high numerical aperture optics, this microscope achieves lateral and axial resolutions that are comparable to the square illumination mode of lattice light-sheet microscopy, but in a user friendly and versatile format. Given this performance, we demonstrate high-resolution imaging of clathrin-mediated endocytosis, vimentin, the endoplasmic reticulum, membrane dynamics, and Natural Killer-mediated cytotoxicity. Furthermore, we image biological phenomena that would be otherwise challenging or impossible to perform in a traditional light-sheet microscope geometry, including cell migration through confined spaces within a microfluidic device, subcellular photoactivation of Rac1, diffusion of cytoplasmic rheological tracers at a volumetric rate of 14 Hz, and large field of view imaging of neurons, developing embryos, and centimeter-scale tissue sections.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707824 | PMC |
| http://dx.doi.org/10.7554/eLife.57681 | DOI Listing |
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