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Although untargeted metabolomic approaches hold great promise for global identification of low molecular weight metabolites in biological samples, deep coverage and confident identification of the metabolites remains challenging due to the great diversity and number of chemical structures, especially in plants. Additionally, there is a need to employ a cell-specific research approach to many physiological and biological responses to specific environmental stimuli. Here, we report an untargeted metabolomic method using Arabidopsis thaliana guard cell samples during response to systemic signals of pathogen attack. We employed a new Acquire X MS data acquisition technology, which uses an iterative fragmentation process to increase level-2 identification of unknown metabolites. We were able to increase the number of identified metabolites and thus the metabolome coverage in Arabidopsis guard cells. This method can be applied to studying metabolomes of other cell types and tissues.
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http://dx.doi.org/10.1007/978-1-0716-0880-7_20 | DOI Listing |
J Biol Chem
March 2025
Molecular Oncology Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, Chennai, Tamil Nadu, India. Electronic address:
Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive form of pancreatic cancer with the worst prognosis. Treating PDAC poses significant challenges, as tumor cells adapt metabolic alterations to thrive in the hypoxic environment created by desmoplasia surrounding the tumor cells. p21-activated kinase (Pak1), a serine-threonine kinase is found to be upregulated in many solid tumors and promotes tumor progression via diverse signalling pathways.
View Article and Find Full Text PDFEnviron Pollut
March 2025
Department of Reproduction, Changzhou Maternity and Child Health Care Hospital, Changzhou Medical Center, Nanjing Medical University, Changzhou, Jiangsu, 213003, PR China. Electronic address:
Pentachlorophenol (PCP) is a pervasive endocrine-disrupting compound present in the environment. Limited research has explored the effects of PCP exposure on gestational diabetes mellitus (GDM), particularly the metabolites-related mechanism. Our study seeks to characterize the interrelationships between PCP exposure, plasma metabolomic markers, and GDM, aiming to elucidate the metabolomic profile mediating PCP-GDM relationship.
View Article and Find Full Text PDFCancer Cell Int
March 2025
Department of Pharmacy, Second Affiliated Hospital of Naval Medical University, No. 415, Fengyang Road, Shanghai, 200003, P. R. China.
Background: Schisandrin B (Sch B) is an active component in Schisandra chinensis exerting anti-cancer effect, but the mechanism is obscure. This study was designed to explore the mechanism of Sch B against colorectal cancer (CRC).
Method: Apparent experiments including cell proliferation, transwell, colony formation, etc.
J Allergy Clin Immunol
March 2025
Channing Division of Network Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA USA.
Background: Inhaled corticosteroids (ICS) are frequently prescribed medications for asthma symptoms, but higher doses can increase risks of adrenal insufficiency through suppression of endogenous cortisol production. Understanding which patients may be at increased risk for developing adrenal suppression related to ICS use may help providers improve treatment regimens for asthma patients; however, the mechanisms underlying ICS-related adrenal insufficiency have not been clarified.
Objective: This study identifies metabolite signatures and biochemical pathways associated with ICS-related adrenal insufficiency in asthma patients.
Int J Food Microbiol
March 2025
Beijing Key Laboratory of Meat Processing Technology, China Meat Research Center, Beijing Academy of Food Sciences, 100068 Beijing, China.
Super-chilling can extend the shelf life of high-oxygen modified atmosphere packaged (MAP) pork from 14 to 56 days compared to conventional chilling storage. The spoilage of raw pork may result from the growth of microorganisms, which ultimately release undesirable metabolites. To investigate this, the microbial and metabolic characteristics of super-chilled MAP pork were determined using 16S rRNA sequencing and untargeted metabolomics based on UHPLC-MS/MS.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!