AI Article Synopsis
- Fluorescent biosensors, especially those based on fluorescent proteins (FPs), are effective for monitoring cellular processes in live organisms due to their selectivity and minimal invasiveness.
- They have been widely used in plant research to track changes in various factors like pH, ion concentration, and redox state.
- The chapter highlights the application of FP-based biosensors in plants, particularly focusing on monitoring intracellular calcium dynamics in Arabidopsis thaliana using a specific genetically encoded Ca indicator.
Article Abstract
Fluorescent biosensors are powerful tools for tracking analytes or cellular processes in live organisms and allowing visualization of the spatial and temporal dynamics of cellular regulators. Fluorescent protein (FP)-based biosensors are extensively employed due to their high selectivity and low invasiveness. A variety of FP-based biosensors have been engineered and applied in plant research to visualize dynamic changes in pH, redox state, concentration of molecules (ions, sugars, peptides, ATP, reactive oxygen species, and phytohormones), and activity of transporters. In this chapter, we briefly summarize reported uses of FP-based biosensors in planta and show simple methods to monitor the dynamics of intracellular Ca in Arabidopsis thaliana using a ratiometric genetically encoded Ca indicator, MatryoshCaMP6s.
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