Two novel actinobacteria, designated strains GY16 and T44, were isolated from the leaves and rhizosphere soil of , respectively. A polyphasic approach was used for determining their taxonomic position. Results of 16S rRNA gene sequence analysis indicated that strain GY16 exhibited highest similarities to subsp. CGMCC 4.1593 (98.82 %), KCTC 19241 (98.76 %), NRRL B-16523 (98.69 %), KACC 20186 (98.69 %) and NBRC 13407 (98.69 %), and strain T44 showed 99.2, 99.1, 99.1 and <98.7 % sequence similarities to CGMCC 4.1452, subsp. DSM 40028, DSM 40539 and other species, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GY16 formed an independent subclade, which indicated that strain GY16 should belong to a potential novel species; and strain T44 was closely related to CGMCC 4.1452, subsp. DSM 40028, DSM 40539 and DSM 40224. However, the multilocus sequence analysis evolutionary distance, average nucleotide identity and DNA-DNA hybridization values between closely related relatives were far from the species-level thresholds. In addition, phenotypic and chemotaxonomic characteristics further confirmed that strains GY16 and T44 belonged to two distinct species. Based on these results, it is concluded that the isolated strains represent novel species within the genus , for which the names sp. nov. (type strain GY16=CICC 24807=KCTC 49326) and sp. nov. (type strain T44=CICC 24819=JCM 33918) are proposed.
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http://dx.doi.org/10.1099/ijsem.0.004556 | DOI Listing |
Surg Pract Sci
December 2023
Faculty of Medicine, Phillipps - University Marburg, Germany.
Background: Lateral heat propagation has been an unavoidable effect of bipolar sealing with the risk of damage to surrounding structures. It is presently unknown whether leaving the perivascular tissue in situ may be advantageous in the sense of an isolation effect.
Material And Methods: Two groups were formed from ex vivo carotid specimens.
Clin Orthop Relat Res
January 2025
Department of Orthopedics, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China.
Background: Bacteremia is sometimes observed in patients with prosthetic joint infection (PJI), and it is associated with a lower likelihood of infection control. However, the prevalence and association of bacteremia in chronic PJI remain unknown.
Questions/purposes: (1) What percentage of patients are diagnosed with bacteremia at the time of hospital admission and before surgery for chronic PJI? (2) What clinical factors are associated with positive blood cultures? (3) To what degree are positive blood cultures associated with infection-free implant survival in patients with chronic PJI?
Methods: This prospective study was conducted at a single academic institution from June 2021 to August 2022.
Int Microbiol
January 2025
State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
Cultivable microbial communities associated with plants inhabiting extreme environments have great potential in biotechnological applications. However, there is a lack of knowledge about these microorganisms from Bryophyllum pinnatum (which survives in severely barren soil) and their ability to promote plant growth. The present study focused on the isolation, identification, biochemical characterization, and potential applications of root endophytic bacteria and rhizosphere bacteria.
View Article and Find Full Text PDFEpidemiol Infect
January 2025
Gastrointestinal Infections and Food Safety (One Health) Division, Clinical and Public Health Group, UK Health Security Agency, London, UK.
In July 2022, a genetically linked and geographically dispersed cluster of 12 cases of Shiga toxin-producing (STEC) O103:H2 was detected by the UK Health Security Agency using whole genome sequencing. Review of food history questionnaires identified cheese (particularly an unpasteurized brie-style cheese) and mixed salad leaves as potential vehicles. A case-control study was conducted to investigate exposure to these products.
View Article and Find Full Text PDFFront Genet
January 2025
Chongqing Engineering Laboratory of Green Planting and Deep Processing of Famous-Region Drug in the Three Gorges Reservoir Region, College of Biology and Food Engineering, Chongqing Three Gorges University, Chongqing, China.
Introduction: P. Y. Li is a plant used to treat respiratory diseases such as pneumonia, bronchitis, and influenza.
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