Comprehensive analysis of , and rearrangements in locally advanced rectal cancer.

Gene rearrangements, such as anaplastic lymphoma kinase (), c-ros oncogene 1 receptor tyrosine kinase (), rearranged during transfection () and neurotrophic receptor tyrosine kinase 1 (), identified in cancer have been indicated to be robust therapeutic targets in lung carcinomas. However, a few studies have focussed on locally advanced rectal cancer (LARC). The discovery of novel gene fusions is also valuable for LARC research. We used mass spectrometry-based assays and RNA sequencing to detect both known , , and rearrangements and novel gene fusions in LARC patients. FusionMap was also used to find gene fusions. None of the , , or gene fusions were detected by mass spectrometry-based assays or RNA sequencing. Three fusion candidates, integrin subunit beta 7 ()-ROS1, lamin A/C ()- and Golgi-associated PDZ and coiled-coil motif containing ()-keratin 8 (), showed relatively high junction-spanning reads by the FusionMap algorithm, but did not pass validation. These results suggest that no , or rearrangements were found in LARC.