Effects of Fe on microbial communities shifts, functional genes expression and nitrogen transformation during the start-up of Anammox process.

Bioresour Technol

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, PR China. Electronic address:

Published: January 2021

In this study, the effect of Fe on the start-up of Anammox process was investigated. Four EGSB reactors were operated with the addition of 0 (R1), 0.04 (R2), 0.08 (R3) and 0.14 (R4) mmol/L Fe, respectively. The results showed that Fe remarkably improved the nitrogen loading rate (NLR) and operation efficiency of the reactor. After 180 days, the influent NH-N concentration in the four reactors was 201.4, 301.8, 343.2, 380.2 mg N/L, and the NLR was 589.3, 877.6, 993.0, 1105.8 mg N/(L·d), respectively. And the nitrogen removal rate (NRR) in R2, R3 and R4 was respectively 1.54, 1.73 and 1.94 times of that in R1. High throughput sequencing revealed that Fe could promote the enrichment of Anammox bacteria Candidatus Brocadia. Moreover, the analysis by qPCR indicated that the abundance of Anammox 16S rRNA gene and the functional gene hzsB increased, which showed a positive correlation with the concentration of Fe.

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http://dx.doi.org/10.1016/j.biortech.2020.124326DOI Listing

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