Transcriptomics in Trichoderma reesei.

Transcriptomics is a powerful technique to study gene expression. The main purpose of transcriptome studies in the filamentous fungus Trichoderma reesei is the analysis of differentially expressed genes as a transcriptional response of the genome to different environmental stimuli or physiological conditions such as sugar availability, nitrogen metabolism, pH response, and oxidative stress, among others. Here we describe the full protocol of RNA sequencing methodology from RNA isolation to data analysis in order to access the T. reesei transcriptome.