Open the Pores: Electroporation for the Transformation of Trichoderma reesei.

Methods Mol Biol

Austrian Centre of Industrial Biotechnology (ACIB) GmbH c/o Research Division Biochemical Technology, Institute of Chemical, Environmental & Bioscience Engineering, TU Wien, Vienna, Austria.

Published: March 2021

During the electroporation of T. reesei, linearized exogenous DNA is absorbed into swollen conidia by an electrical impulse. The advantage of this method is that it is less time-consuming, less expensive, and easier to perform than the classical protoplast transformation while at the same time having a comparable efficiency.

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Source
http://dx.doi.org/10.1007/978-1-0716-1048-0_6DOI Listing

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