Prevalence data of diarrheagenic in the fecal pellets of wild rodents using culture methods and PCR assay.

Wild animals, such as rodents seem to be competent reservoir of bacteria-borne zoonotic diseases which disseminate in human. We investigated the presence of , Shiga toxin-producing (STEC), and in the feces of six category wild rodent species ( and ) captured from different agricultural regions in South Korea. Among them, , which account for 65% of total ( = 52) individuals, are most widely distributed and abundant in various agroecosystems in South Korea. The bacterial identification was performed by cultural and molecular methods. In cultural method, the fecal cultures from 26 individuals formed colonies on -selective EMB agar media. Of them, the fecal cultures from 18 individuals also produced colonies on the Shiga toxin-producing -selective CT-SMAC agar media as well as the EMB agar media. In molecular method, polymerase chain reaction (PCR) was carried out to detect two virulence genes ( and ) of isolated . The amplified dataset of and genes of were sequenced. In this manuscript, and STEC were detected but there were no species. The wild rodents' data would provide important information on reservoirs of those pathogenic bacteria.