Polylysine-modified MXene nanosheets with highly loaded glucose oxidase as cascade nanoreactor for glucose decomposition and electrochemical sensing.

Two-dimensional (2D) nanoreactors with cascade catalytic activity for glucose oxidation and hydrogen peroxide decomposition are prepared via immobilizing glucose oxidase (GOx) on TiC MXene nanosheets. Amino-rich polypeptide, poly-l-lysine (PLL), is applied to modify the ultra-thin TiC MXene nanosheets with a compatible surface for GOx immobilization. The PLL-modified TiC nanosheets possess a positively charged surface and show an excellent GOx loading capacity as high as 50 wt% of the TiC nanosheets. The physically adsorbed enzymes are then cross-linked with the amine groups in the PLL chains to form a robust GOx-PLL network covered on the MXene nanosheets. The GOx-conjugated TiC-PLL (TiC-PLL-GOx) nanosheets showed superior enzymatic activities than the activities of GOx immobilized on an inert porous silica substrate, largely because that the TiC nanosheets can catalyze the decomposition of the toxic intermediate HO generated from the glucose oxidation. Given the excellent electrical conductivity of TiC MXene, the TiC-PLL-GOx nanosheets are further deposited on glassy carbon electrode to construct a high-performance biosensor with a glucose detection limit of 2.6 μM.