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Microprofiling real time nitric oxide flux for field studies using a stratified nanohybrid carbon-metal electrode.
Anal Methods
November 2017
Agricultural and Biological Engineering Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA.
Nitric oxide (NO) is an important signaling molecule that is involved in stress response, homeostasis, host defense, and cell development. In most cells, NO levels are in the femtomolar to micromolar range, with extracellular concentrations being much lower. Thus, real time measurement of spatiotemporal NO dynamics near the surface of living cells/tissues is a major challenge.
View Article and Find Full Text PDFIn Vivo Nitrosative Stress-Induced Expression of a Photolyase Promotes Vibrio cholerae Environmental Blue Light Resistance.
Mol Microbiol
January 2025
Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Bacterial pathogens possess a remarkable capacity to sense and adapt to ever-changing environments. For example, Vibrio cholerae, the causative agent of the severe diarrheal disease cholera, thrives in aquatic ecosystems and human hosts through dynamic survival strategies. In this study, we investigated the role of three photolyases, enzymes that repair DNA damage caused by exposure to UV radiation and blue light, in the environmental survival of V.
View Article and Find Full Text PDFStrain-specific virulence of Perkinsus marinus and related species in Eastern oysters: A comprehensive analysis of immune responses and mortality.
Fish Shellfish Immunol
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Department of Aquatic Life Medicine, College of Ocean and Biosciences, Kunsan National University, Gunsan, 54150, Republic of Korea; Research Institute of Fisheries Science in Offshore Wind Farm (RIFSO), Kunsan National University, 558 Daehakro, Gunsan, 54150, Republic of Korea. Electronic address:
In this study, we investigated the variability in virulence among different strains of Perkinsus marinus and other Perkinsus species in Eastern oysters (Crassostrea virginica), examining the immune responses and mortality rates of oysters exposed to different Perkinsus isolates. Compared with the other assessed strains, P. marinus strain ATCC 50787 was found to induce significantly (P < 0.
View Article and Find Full Text PDFGram Negative Biofilms: Structural and Functional Responses to Destruction by Antibiotic-Loaded Mixed Polymeric Micelles.
Microorganisms
December 2024
Department of Microbiology, Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Akad. G. Bonchev Street, bl. 26, 1113 Sofia, Bulgaria.
Biofilms are a well-known multifactorial virulence factor with a pivotal role in chronic bacterial infections. Their pathogenicity is determined by the combination of strain-specific mechanisms of virulence and the biofilm extracellular matrix (ECM) protecting the bacteria from the host immune defense and the action of antibacterials. The successful antibiofilm agents should combine antibacterial activity and good biocompatibility with the capacity to penetrate through the ECM.
View Article and Find Full Text PDFThe Role of Scavenger Receptor BI in Sepsis.
Int J Mol Sci
December 2024
Department of Pharmacology and Nutritional Sciences, University of Kentucky College of Medicine, Lexington, KY 40536, USA.
Sepsis is a life-threatening condition resulting from a dysregulated host response to infection. Currently, there is no effective therapy for sepsis due to an incomplete understanding of its pathogenesis. Scavenger receptor BI (SR-BI) is a high-density lipoprotein (HDL) receptor that plays a key role in HDL metabolism by modulating the selective uptake of cholesteryl ester from HDL.
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