We demonstrate that plasma membrane biosynthesis and vacuole formation require DNA replication in protoplasts. The replication inhibitor novobiocin inhibited not only DNA replication but also cell enlargement (plasma membrane biosynthesis) and vacuole formation during the enlargement of the protoplasts. After novobiocin treatment prior to vacuole formation, the cell size of protoplasts was limited to 6 μm in diameter and the cells lacked vacuoles. When novobiocin was added after vacuole formation, protoplasts grew with vacuole enlargement; after novobiocin removal, protoplasts were enlarged again. Although cell size distribution of the protoplasts was similar following the 24 h and 48 h novobiocin treatments, after 72 h of novobiocin treatment there was a greater number of smaller sized protoplasts, suggesting that extended novobiocin treatment may inhibit the re-enlargement of protoplasts after novobiocin removal. Our findings demonstrate that novobiocin can control the enlargement of protoplasts due to inhibition of DNA replication.
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| http://dx.doi.org/10.15698/mic2020.11.735 | DOI Listing |
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