AI Article Synopsis
- Most microbes remain uncultured despite advancements in microbial culture, and the effects of medium composition on isolating these microbes are not fully understood.
- High-throughput sequencing (HTS) and culture techniques were used to study microbial communities in marine sediment samples, revealing differences in community composition between the two methods.
- A combination of media was found to culture a greater variety of taxa than any single medium, with nutrient-rich and specific carbon/nitrogen sources supporting fewer taxa, while the type of nitrogen used significantly impacted bacterial isolation.
Article Abstract
Despite recent great advances in microbial culture, most microbes have not yet been cultured, and the impact of medium composition on the isolation of microbes from natural systems has not been elucidated. To optimize media for culturing marine microbes, microbial communities in three sediment samples were described using high-throughput sequencing (HTS) and culture-dependent techniques. HTS revealed communities dominated by , and culture-based methods revealed communities dominated by . Among the total operational taxonomic units (OTUs) from the HTS dataset, 6% were recovered in the culture collection. Four potentially novel bacterial strains belonging to , and were isolated. The combination of media cultured more taxa than any single medium. Nutrient-rich and single-carbon/nitrogen-source media supported the growth of relatively few taxa, and the quality of nitrogen strongly influenced the types of bacteria isolated.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585373 | PMC |
| http://dx.doi.org/10.7717/peerj.10060 | DOI Listing |
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