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Changes in SARS-CoV-2 Spike versus Nucleoprotein Antibody Responses Impact the Estimates of Infections in Population-Based Seroprevalence Studies. | LitMetric

AI Article Synopsis

  • - The study evaluated antibody responses to SARS-CoV-2 in individuals with acute infection and in those post-infection, focusing on the spike (S) protein and nucleocapsid (N) proteins in a Swiss population.
  • - Results showed that while antibody responses to both S and N proteins were similarly sensitive during acute infection, N protein responses declined more rapidly after infection compared to S protein responses.
  • - The research highlighted that using the native trimer form of the S protein in serological assays significantly improved sensitivity in detecting SARS-CoV-2 infections, suggesting it should be prioritized in population studies.

Article Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibody responses to the spike (S) protein monomer, S protein native trimeric form, or the nucleocapsid (N) proteins were evaluated in cohorts of individuals with acute infection ( = 93) and in individuals enrolled in a postinfection seroprevalence population study ( = 578) in Switzerland. Commercial assays specific for the S1 monomer, for the N protein, or within a newly developed Luminex assay using the S protein trimer were found to be equally sensitive in antibody detection in the acute-infection-phase samples. Interestingly, compared to anti-S antibody responses, those against the N protein appear to wane in the postinfection cohort. Seroprevalence in a "positive patient contacts" group ( = 177) was underestimated by N protein assays by 10.9 to 32.2%, while the "randomly selected" general population group ( = 311) was reduced by up to 45% relative to the S protein assays. The overall reduction in seroprevalence targeting only anti-N antibodies for the total cohort ranged from 9.4 to 31%. Of note, the use of the S protein in its native trimer form was significantly more sensitive compared to monomeric S proteins. These results indicate that the assessment of anti-S IgG antibody responses against the native trimeric S protein should be implemented to estimate SARS-CoV-2 infections in population-based seroprevalence studies. In the present study, we have determined SARS-CoV-2-specific antibody responses in sera of acute and postinfection phase subjects. Our results indicate that antibody responses against viral S and N proteins were equally sensitive in the acute phase of infection, but that responses against N appear to wane in the postinfection phase where those against the S protein persist over time. The most sensitive serological assay in both acute and postinfection phases used the native S protein trimer as the binding antigen, which has significantly greater conformational epitopes for antibody binding compared to the S1 monomer protein used in other assays. We believe these results are extremely important in order to generate correct estimates of SARS-CoV-2 infections in the general population. Furthermore, the assessment of antibody responses against the trimeric S protein will be critical to evaluate the durability of the antibody response and for the characterization of a vaccine-induced antibody response.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925109PMC
http://dx.doi.org/10.1128/JVI.01828-20DOI Listing

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