The peroxidase activity of hemin-peptide complexes remains a potential factor in oxidative damage relevant to neurodegeneration. Here, we present the effect of temperature, ionic strength, and pH relevant to pathophysiological conditions on the dynamic equilibrium between high-spin and low-spin hemin-Aβ constructs. This influence on peroxidase activity was also demonstrated using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and dopamine (DA) oxidation rate analyses with increasing ratios of Aβ and Aβ (up to 100 equivalents). Interaction and reactivity studies of aggregated Aβ-hemin revealed enhanced peroxidase activity versus hemin alone. Comparison of the results obtained using Aβ and Aβ amyloid beta peptides revealed marked differences and provide insight into the potential effects of hemin-Aβ on neurological disease progression.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662341 | PMC |
| http://dx.doi.org/10.3390/molecules25215044 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Resveratrol mitigates heat stress-induced testicular injury in rats: enhancing male fertility via antioxidant, antiapoptotic, pro-proliferative, and anti-inflammatory mechanisms.
Naunyn Schmiedebergs Arch Pharmacol
January 2025
Department of Pathology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, 22758, Egypt.
This study investigates the protective effects of resveratrol (RSV) against heat stress (HS)-induced testicular injury in rats. Climate change has exacerbated heat stress, particularly affecting male fertility by impairing testicular function and sexual behavior. A total of 32 rats were allocated into four experimental groups: control, RSV control, HS control, and RSV + HS.
View Article and Find Full Text PDFPeroxidase (POD) Mimicking Activity of Different Types of Poly(ethyleneimine)-Mediated Prussian Blue Nanoparticles.
Nanomaterials (Basel)
December 2024
Diagnostic Nanotools Group, Hospital Vall d'Hebron Institut de Recerca (VHIR), 08035 Barcelona, Spain.
Prussian blue nanoparticles (PBNPs) have been identified as a promising candidate for biomimetic peroxidase (POD)-like activity, specifically due to the metal centres (Fe/Fe) of Prussian blue (PB), which have the potential to function as catalytically active centres. The decoration of PBNPs with desired functional polymers (such as amino- or carboxylate-based) primarily facilitates the subsequent linkage of biomolecules to the nanoparticles for their use in biosensor applications. Thus, the elucidation of the catalytic POD mimicry of these systems is of significant scientific interest but has not been investigated in depth yet.
View Article and Find Full Text PDFThe Effects of Polystyrene Microplastics and Copper Ion Co-Contamination on the Growth of Rice Seedlings.
Nanomaterials (Basel)
December 2024
State Key Laboratory of Environmental Criteria and Risk Assessment, State Environmental Protection Key Laboratory of Regional Eco-Process and Function Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
Microplastics (MPs) are emerging pollutants of global concern, while heavy metals such as copper ions (Cu) are longstanding environmental contaminants with well-documented toxicity. This study investigates the independent and combined effects of polystyrene microplastics (PS-MPs) and Cu on the physiological and biochemical responses of rice seedlings ( L.), a key staple crop.
View Article and Find Full Text PDFExploring the potential of gemcitabine-metal-organic frameworks in combating pancreatic cancer under ketogenic conditions.
BMC Cancer
January 2025
Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Background: Inadequate treatment responses, chemotherapy resistance, significant heterogeneity, and lengthy treatment durations create an urgent need for new pancreatic cancer therapies. This study aims to investigate the effectiveness of gemcitabine-loaded nanoparticles enclosed in an organo-metallic framework under ketogenic conditions in inhibiting the growth of MIA-PaCa-2 cells.
Methods: Gemcitabine was encapsulated in Metal-organic frameworks (MOFs) and its morphology and size distribution were examined using transmission electron microscopy (TEM) and Dynamic light scattering (DLS) with further characterization including FTIR analysis.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!