AI Article Synopsis
- Giardia infections in dogs cause diarrhea and have zoonotic potential, but are often misdiagnosed due to diagnostic challenges.
- This study evaluates the effectiveness of the Speed Giardia immunochromatographic test compared to gold standard methods (enzyme immunoassay and PCR) and traditional microscopy.
- Results show that the Speed Giardia test is significantly more sensitive (86.2%) than microscopy (58.6%) while both methods have 100% specificity, making it a more efficient and reliable option for diagnosing Giardia infections in dogs.
Article Abstract
Background: Infections by protozoans of the genus Giardia are a common cause of diarrhea in dogs. Canine giardiosis constitutes a disease with a zoonotic potential; however, it is often underestimated due to its challenging diagnosis. The objective of the study was to assess the diagnostic performance of an immunochromatographic strip test (Speed Giardia, Virbac, France) comparing it with microscopy (zinc sulfate flotation) by utilizing the combination of an enzyme immunoassay (ProSpecT Giardia EZ Microplate Assay, Oxoid Ltd., UK) and the PCR as the gold standard. A positive result in both ELISA and PCR was set as the gold standard.
Methods: Initially, fecal samples from dogs with clinical signs compatible with giardiosis were tested with the Speed Giardia test and separated into two groups of 50 samples each: group A (positive) and group B (negative). Thereafter, all samples were examined by zinc sulfate centrifugal flotation technique and assayed by the ProSpecT Giardia Microplate Assay and PCR. The performance of the Speed Giardia and zinc sulfate centrifugal flotation tests were calculated estimating sensitivity, specificity, and positive and negative likelihood ratio; the chi-square and McNemar tests were used for the comparison of the two methods.
Results: Giardia cysts were not detected by microscopy in 16 out of the 50 samples (32%) of group A and in none of group B samples. Eight out of 50 samples in group B (16%) were tested positive both with the ProSpecT Giardia Microplate Assay and PCR. Fecal examination with the Speed Giardia test was more sensitive (86.2%) than the parasitological method (58.6%, P < 0.001) while the specificity of both methods was 100%.
Conclusions: The Speed Giardia test is an easy-to-perform diagnostic method for the detection of Giardia spp., which can increase laboratory efficiency by reducing time and cost and decrease underdiagnosis of Giardia spp. infections. This immunochromatographic strip test may be routinely exploited when a rapid and reliable diagnosis is required, other diagnostic techniques are unavailable and microscopy expertise is inefficient. In negative dogs with compatible clinical signs of giardiosis, it is recommended either to repeat the exam or proceed with further ELISA and PCR testing.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607699 | PMC |
| http://dx.doi.org/10.1186/s13071-020-04422-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization of a unique attachment organelle: Single-cell force spectroscopy of trophozoites.
Nanoscale
April 2024
Institute of Medical Microbiology and Hygiene, Saarland University, Homburg, Germany.
Article Synopsis
- The unicellular parasite is responsible for giardiasis, a widespread gastrointestinal illness, and attaches to human intestines using a specialized organelle called the ventral disc.
- Researchers are investigating how this attachment occurs by measuring the detachment characteristics and adhesion forces of individual trophozoites on smooth surfaces using a technique called fluidic force microscopy.
- The study found that the adhesion forces of trophozoites exhibit unique patterns compared to other eukaryotic cells, indicating distinct mechanisms of attachment and detachment at the molecular level.
Diarrheal disease, still a major cause of childhood illness, is caused by numerous, diverse infectious microorganisms, which are differentially sensitive to environmental conditions. Enteropathogen-specific impacts of climate remain underexplored. Results from 15 studies that diagnosed enteropathogens in 64,788 stool samples from 20,760 children in 19 countries were combined.
View Article and Find Full Text PDFDeep learning-enabled imaging flow cytometry for high-speed Cryptosporidium and Giardia detection.
Cytometry A
November 2021
Nanyang Environment and Water Research Institute, Nanyang Technological University, Singapore, Singapore.
Imaging flow cytometry has become a popular technology for bioparticle image analysis because of its capability of capturing thousands of images per second. Nevertheless, the vast number of images generated by imaging flow cytometry imposes great challenges for data analysis especially when the species have similar morphologies. In this work, we report a deep learning-enabled high-throughput system for predicting Cryptosporidium and Giardia in drinking water.
View Article and Find Full Text PDFRapid on-site diagnosis of canine giardiosis: time versus performance.
Parasit Vectors
November 2020
Laboratory of Parasitology and Parasitic Diseases, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124, Thessaloniki, GR, Greece.
Article Synopsis
- Giardia infections in dogs cause diarrhea and have zoonotic potential, but are often misdiagnosed due to diagnostic challenges.
- This study evaluates the effectiveness of the Speed Giardia immunochromatographic test compared to gold standard methods (enzyme immunoassay and PCR) and traditional microscopy.
- Results show that the Speed Giardia test is significantly more sensitive (86.2%) than microscopy (58.6%) while both methods have 100% specificity, making it a more efficient and reliable option for diagnosing Giardia infections in dogs.
Length-dependent disassembly maintains four different flagellar lengths in .
Elife
December 2019
Department of Microbiology and Molecular Genetics, University of California, Davis, Davis, United States.
With eight flagella of four different lengths, the parasitic protist is an ideal model to evaluate flagellar assembly and length regulation. To determine how four different flagellar lengths are maintained, we used live-cell quantitative imaging and mathematical modeling of conserved components of intraflagellar transport (IFT)-mediated assembly and kinesin-13-mediated disassembly in different flagellar pairs. Each axoneme has a long cytoplasmic region extending from the basal body, and transitions to a canonical membrane-bound flagellum at the 'flagellar pore'.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!