Hybrid strains of combine virulence traits of diarrheagenic (DEC) and extraintestinal pathogenic (ExPEC), but it is poorly understood whether these combined features improve the virulence potential of such strains. We have previously identified a uropathogenic (UPEC) strain (UPEC 252) harboring the gene that encodes the adhesin intimin and is located in the locus of enterocyte effacement (LEE) pathogenicity island. The LEE-encoded proteins allow enteropathogenic (EPEC) and enterohemorrhagic (EHEC) to form attaching and effacing (A/E) lesions in enterocytes. We sought to characterize UPEC 252 through whole-genome sequencing and phenotypic virulence assays. Genome analysis unveiled that this strain harbors a complete LEE region, with more than 97% of identity comparing to E2348/69 (EPEC) and O157:H7 Sakai (EHEC) prototype strains, which was functional, since UPEC 252 expressed the LEE-encoded proteins EspB and intimin and induced actin accumulation foci in HeLa cells. Phylogenetic analysis performed comparing 1,000 single-copy shared genes clustered UPEC 252 with atypical EPEC strains that belong to the sequence type 10, phylogroup A. Additionally, UPEC 252 was resistant to the bactericidal power of human serum and colonized cells of the urinary (T24 and HEK293-T) and intestinal (Caco-2 and LS174T) tracts. Our findings suggest that UPEC 252 is an atypical EPEC strain that emerges as a hybrid strain (aEPEC/UPEC), which could colonize new niches and potentially cause intestinal and extraintestinal infections.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550682 | PMC |
| http://dx.doi.org/10.3389/fcimb.2020.00492 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Incidence, In-Hospital and Long-Term Mortality, and Sequelae of Epidermal Necrolysis in Adults.
JAMA Dermatol
December 2024
Service de Dermatologie, Hôpital Henri Mondor, Assistance Publique-Hôpitaux de Paris (AP-HP), Créteil, France.
Article Synopsis
- The study investigates the incidence and mortality rates of epidermal necrolysis (EN), including Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN), analyzing data from adult patients in the French Health System over nine years.
- It found an in-hospital mortality rate of 19% and a postdischarge mortality rate of 15%, leading to an overall mortality of 34% among patients.
- Key factors influencing in-hospital mortality include age, history of cancer, dementia, liver disease, and the severity of EN, with cancer and liver disease also impacting postdischarge mortality.
Validation of the R3-AFP model for risk prediction of HCC recurrence after liver transplantation in the SiLVER randomized clinical trial.
Liver Transpl
January 2025
Department of Hepatology, Medical Liver Transplant Unit, Hospital Henri Mondor AP-HP, University of Paris-Est Créteil (UPEC), Créteil, France.
Explant-based models for assessing HCC recurrence after liver transplantation serve as the gold standard, guiding post-liver transplantation screening and immunosuppression adjustment. Incorporating alpha-fetoprotein (AFP) levels into these models, such as the novel R3-AFP score, has notably enhanced risk stratification. However, validation of these models in high-evidence data is mandatory.
View Article and Find Full Text PDFComparison of incidence, causes and prognosis of adult and paediatric epidermal necrolysis: a French population-based study.
Br J Dermatol
October 2024
Service de Dermatologie, Hôpital Henri Mondor, AP-HP, Créteil, France.
Article Synopsis
- Epidermal necrolysis (EN) includes severe conditions like Stevens-Johnson syndrome and is primarily drug-induced, with notable differences in incidence and outcomes between children and adults.
- A study analyzed data from the French Health System to compare EN cases in both demographics, examining incidence, suspected drug exposure, and mortality rates.
- Results showed that EN is less common in children (1.5 cases/million) compared to adults (2.6 cases/million), with children having a lower rate of drug exposure before onset and significantly lower mortality (1.4% vs. 19.4%).
Research on Membranes and Their Associated Processes at the Université Paris-Est Créteil: Progress Report, Perspectives, and National and International Collaborations.
Membranes (Basel)
February 2023
ICMPE, CNRS, Université Paris-Est Créteil, UMR 7182, 2 Rue Henri Dunant, 94320 Thiais, France.
Research on membranes and their associated processes was initiated in 1970 at the University of Paris XII/IUT de Créteil, which became in 2010 the University Paris-Est Créteil (UPEC). This research initially focused on the development and applications of pervaporation membranes, then concerned the metrology of ion-exchange membranes, then expanded to dialysis processes using these membranes, and recently opened to composite membranes and their applications in production or purification processes. Both experimental and fundamental aspects have been developed in parallel.
View Article and Find Full Text PDFIn vitro activities of 8 antifungal agents against geophilic dermatophyte isolates.
Mycoses
February 2022
Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Background: Members of the Nannizzia gypsea complex are globally the most common geophilic dermatophytes which cause infection in animals and human. Although the susceptibility patterns of anthropophilic or zoophilic dermatophyte species to antifungal agents are well documented, the effectiveness of such drugs against geophilic species have rarely been explored.
Objectives: This study was aimed to evaluate the in vitro antifungal activity of common and new antifungals against a set of environmental and clinical geophilic dermatophyte isolates.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!