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Potential functions of embryonic cardiac macrophages in angiogenesis, lymphangiogenesis and extracellular matrix remodeling. | LitMetric

AI Article Synopsis

  • The study focuses on understanding cardiac tissue macrophages (cTMs) during fetal development, particularly their populations and functions based on specific surface markers.
  • Immunostaining of murine fetal hearts revealed that cTMs are primarily located in the subepicardial space and interact with newly formed blood and lymphatic vessels.
  • Three distinct subpopulations of cTMs were identified, showing different gene expression levels related to angiogenesis, lymphangiogenesis, and extracellular matrix remodeling, indicating their diverse roles in heart development.

Article Abstract

The role of cardiac tissue macrophages (cTMs) during pre- and postnatal developmental stages remains in many aspects unknown. We aimed to characterize cTM populations and their potential functions based on surface markers. Our in situ studies of immunostained cardiac tissue specimens of murine fetuses (from E11to E17) revealed that a significant number of embryonic cTMs (phenotyped by CD45, CD68, CD64, F4/80, CD11b, CD206, Lyve-1) resided mostly in the subepicardial space, not in the entire myocardial wall, as observed in adult individuals. cTMs accompanied newly developed blood and lymphatic vessels adhering to vessel walls by cellular processes. A subpopulation of CD68-positive cells was found to form accumulations in areas of massive apoptosis during the outflow tract remodeling and shortening. Flow cytometry analysis at E14 and E17 stages revealed newly defined three subpopulations:CD64, CD64CD206-and CD64CD206+. The levels of mRNA expression for genes related to regulation of angiogenesis (VEGFa, VEGFb, VEGFc, bFGF), lymphangiogenesis (VEGFc) and extracellular matrix (ECM) remodeling (MMP13, Arg1, Ym1/Chil3, Retlna/FIZZ1) differed among the selected populations and/or embryonic stages. Our results demonstrate a diversity of embryonic cTMs and their tissue-specific locations, suggesting their various potential roles in regulating angiogenesis, lymphangiogenesis and ECM remodeling.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847984PMC
http://dx.doi.org/10.1007/s00418-020-01934-1DOI Listing

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