The increasing interest of the biopharmaceutical industry to exploit plants as a commercially viable production system is demanding the development of new strategies to maximize product recovery. Aqueous two-phase systems (ATPSs) are a primary recovery technique that has shown great potential for the efficient extraction and purification of biological products, from organelles to proteins and low-molecular-weight compounds. The evaluation of different system parameters upon the partitioning behavior can provide the conditions that favor the concentration of contaminants and the desired target protein in opposite phases. The protocols described here provide the basic strategy to explore the use of ATPSs for the isolation and partial purification of native and recombinant proteins from plants and plant-derived extracts.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-0775-6_7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mobilization of DNAPL lenses in heterogeneous aquifers using shear-thinning PEO polymers: Experimental and numerical study.
Water Res
December 2024
BRGM (French Geological Survey), Orléans 45060, France.
Polymer solution injection has emerged as a promising method for the remediation of NAPL (non-aqueous phase liquids)-contaminated aquifers. This technique enhances recovery efficiency by modifying viscous forces, stabilizing the displacement front, and minimizing channeling effects. However, there remains a significant gap in understanding the behavior of polymer solutions, particularly those with different molecular weights (MW), for mobilizing DNAPL (dense non-aqueous phase liquids) trapped in heterogeneous aquifers, especially within low-permeability layers.
View Article and Find Full Text PDFPermeability-Engineered Compartmentation System-Enabled Digital PCR (PECS-dPCR): A Digital Platform toward Multistep Biomolecular Assays.
Anal Chem
December 2024
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Droplet-based digital PCR has emerged as a powerful platform for nucleic acid-based detection. However, the formation of droplet compartments and the subsequent amplification process in oil present significant drawbacks: instability under harsh thermal conditions, high background fluorescent noise inside droplets, and major difficulty in supporting multistep assays. Alternatively, droplets made of a hydrogel, or other advanced materials, have been adopted and demonstrate promising improvement over conventional droplet-based platforms.
View Article and Find Full Text PDFA generalized model for predicting different morphologies of bacterial swarming on a porous solid surface.
Soft Matter
December 2024
Department of Chemical Engineering, Indian Institute of Technology Madras, Chennai, Tamil Nadu 600036, India.
In this study, we develop a comprehensive two-phase model to analyze the dynamics of bacterial swarming on porous substrates. The two distinct phases under consideration are the cell and aqueous phases. We use the thin-film approximation, as the characteristic height of the swarm is significantly lower than its characteristic radius.
View Article and Find Full Text PDFOn the compatibility of the Madrid-2019 force field for electrolytes with the TIP4P/Ice water model.
J Chem Phys
December 2024
Dpto. Química Física I, Fac. Ciencias Químicas, Universidad Complutense de Madrid, 28040 Madrid, Spain.
The Madrid-2019 force field was recently developed to perform simulations of electrolytes in water. The model was specifically parameterized for TIP4P/2005 water and uses scaled charges for the ions. In this work, we test the compatibility of the Madrid-2019 force field with another water model: TIP4P/Ice.
View Article and Find Full Text PDFOn the phase behaviors of CH4-CO2 binary clathrate hydrates: Equilibrium with aqueous phase.
J Chem Phys
December 2024
Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Osaka 560-8531, Japan.
We explore the solubilities of guest CH4 and/or CO2 in the aqueous state coexisting with the corresponding hydrate. The equilibrium conditions are estimated by calculating the chemical potentials of water and guest species in the hydrate on the basis of a statistical mechanical theory using pairwise intermolecular potentials. This requires the least computational cost while covering a wide range of temperature, pressure, and composition of guest species, even for the binary hydrate.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!