Molecular analysis and bioinformatic characterization of cooper, zinc-superoxide dismutase (Cu/Zn-sod) gene of Caiman latirostris.

Mol Biol Rep

Cátedra de Toxicología, Farmacología y Bioquímica Legal, FBCB-UNL, Ciudad Universitaria, CC 242 Paraje El Pozo S/N, 3000, Santa Fe, Argentina.

Published: November 2020

Superoxide dismutase (SOD) is an antioxidant enzyme that acts as a component of first-line defense system against reactive oxygen species (ROS). Copper/Zinc superoxide dismutase (Cu/Zn-SOD) is one of the isoforms of SOD enzyme and is sensitive to the exposure of different environmental factors, in different species and tissues. Caiman latirostris is one of the two crocodilian species living in Argentina and no information is available on the molecular and biochemical characteristics of the Cu/Zn-sod gene in this species. In the present work, we reported the presence of the Cu/Zn-sod gene in C. latirostris, the nucleotide and amino acid sequences, the modelled protein structure, evolutionary distance among species and tissue specific expression patterns. Cu/Zn-sod gene was 620 bp open reading frame in length and encoded 178 amino acids. The nucleotide sequences of C. latirostris shared high similarity with the Cu/Zn-sod genes of other crocodilian species, so it showed to be highly conserved. PCR analysis showed that Cu/Zn-sod gene was expressed in all the tissues examined (liver, gonads, spleen, heart, and whole blood), suggesting a constitutively expressed gene in these tissues. This study allows further investigation into the structure-activity relationship and the mechanism of action of Cu/Zn-SOD, besides exploring the functional breadth and possible alteration factors, including xenobiotics.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11033-020-05937-yDOI Listing

Publication Analysis

Top Keywords

cu/zn-sod gene
20
cu/zn-sod
8
dismutase cu/zn-sod
8
caiman latirostris
8
superoxide dismutase
8
crocodilian species
8
gene
6
species
6
molecular analysis
4
analysis bioinformatic
4

Similar Publications

This experiment investigated the effects of dietary supplementation with nano-composites of copper and carbon (NCCC) on antioxidants, immune functions, and the cecum microbiota of weaned Ira white rabbits. A total of 240 weaned 35-day-old Ira white rabbits were randomly allocated to five dietary treatments ( = 6 per treatment, each replicate consisted of eight rabbits) that included the control group (CON) with a basal diet, the SAL group with 60 mg/kg salinomycin (SAL) in addition to the basal diet, and the NCCC I, II, III groups, which were supplemented with 50, 100, and 200 mg/kg NCCC, respectively, in addition to the basal diet. The test lasted for 28 d.

View Article and Find Full Text PDF

Astragaloside IV can mitigate heat stress-induced tissue damage through modulation of the Keap1-Nrf2 signaling pathway in grass carp (Ctenopharyngodon idella).

Fish Shellfish Immunol

February 2025

Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan, Guangdong, 528225, China. Electronic address:

This study investigated the potential protective effect of AS-IV against heat stress-induced tissue damage in grass carp (Ctenopharyngodon idella). Grass carp were injected intraperitoneally with 0, 2, 4, and 8 mg/kg of AS-IV for three consecutive days, and then subjected to heat stress (35 ± 0.5 °C); thereafter, histopathological analyses of the liver and spleen were performed at 0, 6, 24, and 48 h, respectively.

View Article and Find Full Text PDF

Pesticides often exist as complex mixtures in soil environments, yet the toxicity of these combinations has not been thoroughly investigated. In light of this, the current study aimed to assess the enzymatic activity and gene expression responses in the earthworm Eisenia fetida when exposed to a mixture of beta-cypermethrin (BCY) and triadimefon (TRI). The findings revealed that co-exposure to BCY and TRI triggered acute synergistic toxicity in E.

View Article and Find Full Text PDF

Impacts of Micro/Nanoplastics Combined with Graphene Oxide on Seeds: Insights into Seedling Growth, Oxidative Stress, and Antioxidant Gene Expression.

Plants (Basel)

December 2024

Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China.

Global pollution caused by micro/nanoplastics (M/NPs) is threatening agro-ecosystems, compromising food security and human health. Also, the increasing use of graphene-family nanomaterials (GFNs) in agricultural products has led to their widespread presence in agricultural systems. However, there is a large gap in the literature on the combined effects of MNPs and GFNs on agricultural plants.

View Article and Find Full Text PDF

Superoxide dismutase (SOD) plays important roles in the balance of oxidation and antioxidation in body mostly by scavenging superoxide anion free radicals (O). Previously, we reported a novel Cu/Zn SOD from jellyfish Cyanea capillata, named CcSOD1, which exhibited excellent SOD activity and high stability. TAT peptide is a common type of cell penetrating peptides (CPPs) that efficiently deliver extracellular biomacromolecules into cytoplasm.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!