AI Article Synopsis
- Osteosarcoma (OSA) is a rare cancer, but its occurrence is 27 times more common in dogs than in humans, and the D-17 cell line is often used for testing anticancer therapies.
- The study aimed to assess the baseline gene expression in D-17 related to immune response and cell cycle regulation, but some gene expressions varied across experiments.
- Findings revealed that D-17 cells show significant gene expression related to innate immune responses and can trigger pro-inflammatory reactions when exposed to infectious stressors, offering insights for developing new treatments.
Article Abstract
Osteosarcoma (OSA) is a rare cancer both in human and dog although the incidence rate in dogs is 27 times higher than in human. Many studies employed D-17 as cell line for in vitro test to evaluate conventional anticancer therapies; however, little is known about D-17 cell line. The aim of our study was to evaluate the basal level of gene expression of pivotal molecules in the innate immune response and cell cycle regulation and to establish the ability of this cell line to react to infective stressor. , , , , , and were expressed in an inconsistent manner among experiments. The other genes under study were expressed in all samples. showed ability to penetrate D-17 causing pro-inflammatory response. Our results outline the expression in D-17 of important genes involved in innate immune response. These results provide important data on D-17 basal gene expression profile useful for in vitro preliminary evaluation of new therapeutic approaches.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693922 | PMC |
| http://dx.doi.org/10.3390/ani10111981 | DOI Listing |
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