[Intraspecific Variation in Growth and Alkaline Phosphatase Activity of Strains in Response to Different Phosphorus Concentrations and Sources].

Huan Jing Ke Xue

Department of Ecology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.

Published: September 2020

The cyanobacterial species are ubiquitous in tropical regions, and its successful invasion into temperate zones has been partially attributed to its ability of survival in low P availability and the existence of multiple ecotypes. To explore the physiological response of different strains to phosphorus fluctuations, four strains of isolated from the Zhenhai Reservoir were used to investigate their growth and alkaline phosphatase (ALP) activity at different inorganic phosphorus (Pi) concentrations (HP=7.13 mg ·L, MP=0.64 mg ·L, LP=0.03 mg ·L) and different phosphorus forms [dipotassium hydrogen phosphate (KHPO), sodium pyrophosphate (KP O), sodium polyphosphate (KPO), D-glucose-6-phosphate (D-G-6-P), adenosine triphosphate (ATP), cyclic adenosine monophosphate (cAMP)]. Four strains showed a similar growth response to phosphate changes: their biomass increased with an increase in Pi concentrations, while the ALP activity showed the opposite trend. The ALP activity of N8 was significantly lower than that of other three strains, regardless of inorganic phosphorus concentrations, suggesting that this strain had a higher adaptability to phosphorus fluctuations. When cultured with different phosphorus forms, the biomass of N8 and N9 in three dissolved inorganic phosphorus (DIP) compounds were significantly higher than those in three dissolved organic phosphorus (DOP) compounds, with the maximum and minimum specific growth rate in KHPOand ATP treatments, respectively. preferred DIP although they can also utilize DOP to sustain its growth. Under the DOP conditions, the ALP activity of N8 in the ATP treatment was significantly higher than that in the other two organic phosphorus compounds, while we did not observe similar results in N9, indicating that strain N8 was more sensitive to DIP deficiency. Our results showed an intraspecific variation within strains from the same reservoir. Compared with the other strains, strain N8 represented better adaptability to phosphorus fluctuations and DIP deficiency. Variations within strains may make this species more adaptable to environmental changes and enhance its competitive advantage.

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http://dx.doi.org/10.13227/j.hjkx.201912180DOI Listing

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