AI Article Synopsis
- The study examines how a high concentration of di(2-ethylhexyl) phthalate (DEHP) influences esterase activity in a specific fungus during liquid fermentation when using calcium nitrate as a nitrogen source.
- It found that higher DEHP concentrations led to increased esterase activity, with the fungus producing five distinct esterase isoforms with varying molecular weights compared to when grown in a glucose medium.
- The results suggest that this fungus has significant potential for bioremediation of sites contaminated with DEHP and similar phthalates due to its enhanced degradation capabilities.
Article Abstract
In this study, the induction of esterase activity during the degradation of a high concentration of di(2-ethylhexyl) phthalate (DEHP) (1500 mg l) by was investigated using Ca(NO) as nitrogen source under liquid fermentation conditions. Assessments of esterase activities through biochemical tests and zymographic assays, as well as fungal growth were studied. A high concentration of DEHP increased esterase activity in , which produces five esterase isoforms (26.4, 31.7, 43, 73.6 and 125 kDa), which were different in abundance and molecular weight to those produced constitutively in glucose-containing medium (control medium). showed higher and values in DEHP-containing medium than those observed in the control medium. has great potential for use in the restoration of sites contaminated with high concentrations of DEHP and even of other phthalates with less complex structures.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588571 | PMC |
| http://dx.doi.org/10.1007/s13205-020-02476-y | DOI Listing |
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