AI Article Synopsis
- SNAP technology uses benzyl-guanine (BG) derivatives for protein labeling, but synthesizing and purifying these substrates can be time-consuming and expensive.
- The proposed method modifies the BG-substrate by incorporating an azide group, enhancing its effectiveness and stability during enzymatic reactions.
- This approach not only improves the self-labeling process but also has significant potential for various biotechnological applications through compatibility with further conjugation techniques.
Article Abstract
SNAP- is a powerful technology for the labelling of protein/enzymes by using benzyl-guanine (BG) derivatives as substrates. Although commercially available or ad hoc produced, their synthesis and purification are necessary, increasing time and costs. To address this limitation, here we suggest a revision of this methodology, by performing a , by using a BG-substrate containing an azide group appropriately distanced by a spacer from the benzyl ring. The SNAP- and its relative thermostable version (OGT- ) proved to be very active on this substrate. The stability of these upon enzymatic reaction makes possible the exposition to the solvent of the azide-moiety linked to the catalytic cysteine, compatible for the subsequent conjugation with DBCO-derivatives by azide-alkyne Huisgen cycloaddition. Our studies propose a strengthening and an improvement in terms of biotechnological applications for this self-labelling .
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599001 | PMC |
| http://dx.doi.org/10.1080/14756366.2020.1841182 | DOI Listing |
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