Among four mycobacteria, , , BCG and () , we established a silkworm infection assay with . When silkworms (fifth-instar larvae, = 5) were infected through the hemolymph with (7.5 × 10 CFU/larva) and bred at 37 °C, they all died around 40 h after injection. Under the conditions, clarithromycin and amikacin, clinically used antimicrobial agents, exhibited therapeutic effects in a dose-dependent manner. Furthermore, five kinds of microbial compounds, lariatin A, nosiheptide, ohmyungsamycins A and B, quinomycin and steffimycin, screened in an in vitro assay to observe anti- activity from 400 microbial products were evaluated in this silkworm infection assay. Lariatin A and nosiheptide exhibited therapeutic efficacy. The silkworm infection model with is useful to screen for therapeutically effective anti- antibiotics.
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| http://dx.doi.org/10.3390/molecules25214971 | DOI Listing |
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