AI Article Synopsis
- A new strategy using polyethyleneimine (PEI) improves the sensitivity of gold nanoparticle-based lateral flow sensors (AuNP-LFS) by aiding in the controlled growth of copper nanoshells on the nanoparticles.
- This method helps regulate the shape and size of the resulting core-shell structures, leading to better consistency in results and fewer false positives.
- The enhanced sensors showed extremely high sensitivity for detecting HIV and E. coli, and were successfully integrated into a commercial kit for diagnosing SARS-CoV-2 antibodies.
Article Abstract
A polyethyleneimine (PEI)-assisted copper in-situ growth (CISG) strategy was proposed as a controlled signal amplification strategy to enhance the sensitivity of gold nanoparticle-based lateral flow sensors (AuNP-LFS). The controlled signal amplification is achieved by introducing PEI as a structure-directing agent to regulate the thermodynamics of anisotropic Cu nanoshell growth on the AuNP surface, thus controlling shape and size of the resultant AuNP@Cu core-shell nanostructures and confining free reduction and self-nucleation of Cu for improved reproducibility and decreased false positives. The PEI-CISG-enhanced AuNP-LFS showed ultrahigh sensitivities with the detection limits of 50 fg mL for HIV-1 capsid p24 antigen and 6 CFU mL for Escherichia coli O157:H7. We further demonstrated its clinical diagnostic efficacy by configuring PEI-CISG into a commercial AuNP-LFS detection kit for SARS-CoV-2 antibody detection. Altogether, this work provides a reliable signal amplification platform to dramatically enhance the sensitivity of AuNP-LFS for rapid and accurate diagnostics of various infectious diseases.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7575433 | PMC |
| http://dx.doi.org/10.1016/j.bios.2020.112753 | DOI Listing |
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