The utility of COI DNA barcodes in species delimitation is explored as well as life stage associations of five closely related species: (Tokunaga, 1938), (Lundström, 1915), Wang, Liu et Paasivirta, 2007, Sæther et Wang, 1996, and (Wen, Zhou et Rong, 1994). Results revealed distinctly larger interspecific than intraspecific divergences and indicated a clear "barcode gap". In total, 42 COI barcode sequences including 16 newly generated DNA barcodes were applied to seven Barcode Index Numbers (BINs). A neighbor-joining (NJ) tree comprises five well-separated clusters representing five morphospecies. Comments on how to distinguish the larvae of and are provided.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7572518PMC
http://dx.doi.org/10.3897/zookeys.957.54668DOI Listing

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