AI Article Synopsis
- Female fertility is significantly affected by the uterus's ability to identify and combat microbial infections, which can cause inflammation in the endometrium.
- This study used RNA-seq technology to explore how PPARγ ligands influence gene expression and alternative splicing in pig endometrium during inflammation triggered by LPS.
- The research discovered multiple differentially expressed genes and alternative splicing events related to LPS and PPARγ treatments, paving the way for future investigations into the role of PPARγ in pig reproductive health.
Article Abstract
Female fertility depends greatly on the capacity of the uterus to recognize and eliminate microbial infections, a major reason of inflammation in the endometrium in many species. This study aimed to determine the in vitro effect of peroxisome proliferator-activated receptor gamma (PPARγ) ligands on the transcriptome genes expression and alternative splicing in the porcine endometrium in the mid-luteal phase of the estrous cycle during LPS-stimulated inflammation using RNA-seq technology. The endometrial slices were incubated in vitro in the presence of LPS and PPARγ agonists-PGJ2 or pioglitazone and antagonist-T0070907. We identified 222, 3, 4, and 62 differentially expressed genes after LPS, PGJ2, pioglitazone, or T0070907 treatment, respectively. In addition, we detected differentially alternative spliced events: after treatment with LPS-78, PGJ2-60, pioglitazone-52, or T0070907-134. These results should become a basis for further studies explaining the mechanism of PPARγ action in the reproductive system in pigs.
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