RNA interference (RNAi) is an important tool for gene function studies in insects, especially in non-model insects. This technology is also being developed for pest control. However, variable RNAi efficiency among insects is limiting its use in insects. Systemic RNAi in requires systemic RNA interference defective protein 1 (. The expression of Ce in insect cell lines was shown to improve RNAi. However, the mechanisms through which this double-stranded RNA (dsRNA) transporter improves RNAi efficiency in insects is not known. We stably expressed in two cell lines, Sf9 and Sf17 cells derived from ovary and midgut, respectively. Expression of enhanced RNAi efficiency in ovarian Sf9 cells, but not in midgut Sf17 cells. Reduced accumulation of dsRNA in late endosomes and successful processing dsRNA to siRNA contribute to enhanced RNAi efficiency in Sf9 cells. Transgenic expressing were produced and tested for RNAi efficiency. RNAi efficiency enhancement due to expression showed tissue specificity. Compared to RNAi efficiency in wild-type expressing transgenic showed a significant improvement of RNAi in tissues such as Verson's glands. In contrast, no improvement in RNAi was observed in tissues such as midgut. The cell-type specific and tissue-specific enhancement of RNAi efficiency by in provides valuable information for improving RNAi in insects such as those belonging to order Lepidoptera where RNAi is variable and inefficient.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354605 | PMC |
http://dx.doi.org/10.1080/15476286.2020.1842632 | DOI Listing |
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