Assessment of acaricidal activity of nanoscale ZnO encapsulated piperine formulation against .

This study aims to synthesise and evaluate the acaricidal activity of nanoscale zinc oxide piperine formulation (NZPF) against ticks. NZPF was prepared by using zinc oxide nanoparticles (ZnONPs) and piperine by employing encapsulation technique; characterised by UV spectroscopy, Fourier transformed infrared analysis, X-ray diffraction, dynamic light scattering, zetapotential and scanning electron microscopy. Acaricidal activity of the NZPF on was evaluated using larval packet test (LPT) and adult immersion test (AIT). LPT against larvae showed an LC at 1 mg/l for NZPF followed by 2 and 3 mg/l for ZnONPs and piperine, respectively. AIT against showed an LC at concentration of 3 mg/l for NZPF followed by 6 mg/l for ZnONPs and 7 mg/l for piperine. In both LPT and AIT, LC values of ZnONPs and NZPF were significantly lower compared to deltamethrin. NZPF showed significant ovulation inhibitory activity with lower IC and IC values compared to ZnONPs and piperine. NZPF has been proved to be the better alternative to routine chemical acaricides for control of tick infestation of cattle in the wake of acaricidal resistance, but safety issues need to be addressed before clinical application.