Mesenchymal stem cells (MSCs) are at the forefront as therapeutic tools for an extensive number of tissue engineering and regenerative medicine applications. MSC differentiation properties have been extensively studied by this laboratory and many others. The generation and validation of potency assays would be a valuable tool for the study of cellular properties relevant for applications. We have developed a unique system, we call the Habitat assay, in which porous ceramic cube carrier loaded with human bone marrow (BM)-MSCs (hMSCs) is subcutaneously implanted into immune-compromised mice. These cells have the capacity to create bone tissue and reconstitute the hematopoietic microenvironment within the "Habitat." These donor-derived hMSCs form bone structures by 3-4 weeks and associate as perivascular MSCs. In this study, we have extensively analyzed data generated with the habitat (ceramic cube assay) using cells derived from 117 hMSC-donors (iliac aspiration); this analysis provides a validation of the platform as a way to study the effect of several variables involved in the generation of the bony Habitat. These studies show that passage number and the age of the hMSC donor influence the sequence of bone formation within the Habitat. These variables have been shown to have an effect on properties of MSCs; in this study, for the first time, we show these effects to be important on an setting.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759284 | PMC |
| http://dx.doi.org/10.1089/ten.TEA.2020.0178 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An overview of the use of cutting-edge artificial intelligence (AI) modeling to produce synthetic medical data (SMD) in decentralized clinical machine learning (ML) for ovarian cancer(OC) and ovarian lymphoma(OL).
J Ultrasound
January 2025
, Costa Contina street n. 19, 66054, Vasto, Chieti, Italy.
Aim: o point out how novel analysis tools of AI can make sense of the data acquired during OL and OC diagnosis and treatment in an effort to help improve and standardize the patient pathway for these disease.
Material And Methods: ultilizing programmed detection of heterogeneus OL and OC habitats through radiomics and correlate to imaging based tumor grading plus a literature review.
Results: new analysis pipelines have been generated for integrating imaging and patient demographic data and identify new multi-omic biomarkers of response prediction and tumour grading using cutting-edge artificial intelligence (AI) in OL and OC.
regulates carbohydrate metabolic functions of the gut microbiome in C57BL/6 mice.
Gut Microbes
December 2025
Department of Microbiology, Immunology & Molecular Genetics, University of Texas Health San Antonio, San Antonio, TX, USA.
The probiotic impact of microbes on host metabolism and health depends on both host genetics and bacterial genomic variation. is the predominant human gut commensal emerging as a next-generation probiotic. Although this bacterium exhibits substantial intraspecies diversity, it is unclear whether genetically distinct strains might lead to functional differences in the gut microbiome.
View Article and Find Full Text PDFStable Soil Biota Network Enhances Soil Multifunctionality in Agroecosystems.
Glob Chang Biol
January 2025
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.
Unraveling how agricultural management practices affect soil biota network complexity and stability and how these changes relate to soil processes and functions is critical for the development of sustainable agriculture. However, our understanding of these knowledge still remains unclear. Here, we explored the effects of soil management intensity on soil biota network complexity, stability, and soil multifunctionality, as well as the relationships among these factors.
View Article and Find Full Text PDFDisentangling Effects of Vegetation Structure and Physiology on Land-Atmosphere Coupling.
Glob Chang Biol
January 2025
Department of Biogeochemical Integration, Max Planck Institute for Biogeochemistry, Jena, Germany.
Terrestrial vegetation is a key component of the Earth system, regulating the exchange of carbon, water, and energy between land and atmosphere. Vegetation affects soil moisture dynamics by absorbing and transpiring soil water, thus modulating land-atmosphere interactions. Moreover, changes in vegetation structure (e.
View Article and Find Full Text PDFGut microbiota shifts in hepatitis B-related portal hypertension after transjugular intrahepatic portosystemic shunt: Mechanistic and clinical implications.
World J Gastroenterol
January 2025
Department of Infectious Diseases, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian Province, China.
In this article, we provide commentary on the recent article by Zhao . We focus on the shifts in the gut microbiota of patients with hepatitis B virus (HBV)-associated cirrhosis/portal hypertension (PH) following transjugular intrahepatic portosystemic shunt (TIPS) and the implications for understanding the mechanisms, diagnosis, and treatment. By comparing the gut microbiota composition and dynamic changes before and after TIPS in patients with and without hepatic encephalopathy, the authors found an increase in non-probiotic bacteria in those who developed hepatic encephalopathy post-TIPS, with species present only in the hepatic encephalopathy group.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!