AI Article Synopsis
- The study focuses on improving backcross breeding using marker-assisted techniques to enhance the recovery of the recurrent parent genome (RPGR), which helps eliminate undesired genetic traits while retaining beneficial ones.
- A specific cross was made between the Malaysian rice variety Putra-1, which is high-yield but susceptible to bacterial leaf blight (BLB), and the BLB-resistant variety IRBB60, utilizing various molecular markers for effective selection.
- The results showed a high RPGR percentage in the progenies, with several lines exhibiting resistance to BLB, leading to recommendations for these new lines as viable rice varieties for commercial farming.
Article Abstract
The most vital aspect of marker-assisted backcross breeding is the recurrent parent genome recovery. This enables the selection of only parents with recovered recipient/recurrent parent genome in addition to the targeted genes. The recurrent parent genome recovery (RPGR) ensures that non-desirable genomic segments are removed while the gene of interest is sustained in the recombined progenies without further segregations. This work was aimed at quantifying the RPGR of backcross populations with introgression of bacterial leaf blight resistance genes. Putra-1, a Malaysian elite variety, high yielding with inherent resistance to blast but susceptible to bacterial leaf blight (BLB), was crossed with IRBB60 which is resistant to BLB disease. The IRBB60 has four resistance genes-, , and . Tightly linked polymorphic functional and SSR markers were used for foreground selection at every stage of backcrossing to select progenies with introgressed target genes. Background selection was done to quantify the percentage of RPGR in the selected lines using 79 confirmed polymorphic microsatellites. Result obtained showed that the percentage of RPGR was 80.11% at BCF, 95.30% at BCF and 95.9% at BCF. The introgression of , , and resistance genes were faster through the adopted marker-assisted backcross breeding compared to what could be obtained through conventional breeding. All the 16 selected lines displayed resistance to BLB with three lines showing high resistance to the disease. The blast resistance contained in the genetic background of Putra-1 was also sustained in all the selected lines. The newly developed lines were recommended as new rice varieties for commercial cultivation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7690635 | PMC |
| http://dx.doi.org/10.3390/plants9111411 | DOI Listing |
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