The quorum quenching (QQ) potential of three pentacyclic triterpenoids, glycyrrhetinic acid (GRA), ursolic acid (UA) and betulinic acid (BA), representing distinct groups of compounds, was evaluated. Violacein production by and pyocyanin production by were severely affected by GRA, UA and BA, suggesting a perturbation of N-acyl homoserine lactone (ASL) based signaling. Molecular docking analysis revealed a possible interaction between ASL-synthase and ASL-dependent transcriptional activator homologs from and with common binding pockets for GRA, UA and BA. The triterpenoids inhibited biofilm formation by and affected the overall structure of biofilms. When administered in combination, two of the three molecules fostered antibiotic action against biofilms, widening the scope for developing novel combinations against the pathogen.
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