The synthetic polymer, polyallylamine hydrochloride (PAA), is found in a variety of applications in biotechnology and medicine. It is used in gene and siRNA transfer, to form microcapsules for targeted drug delivery to damaged and tumor cells. Conventional chemotherapy often does not kill all cancer cells and leads to multidrug resistance (MDR). Until recently, studies of the effects of PAA on cells have mainly focused on their morphological and genetic characteristics immediately or several hours after exposure to the polymer. The properties of the cell progeny which survived the sublethal effects of PAA and resumed their proliferation, were not monitored. The present study demonstrated that treatment of immortalized Chinese hamster cells CHLV-79 RJK sensitive (RJK) and resistant (RJKEB) to ethidium bromide (EB) with cytotoxic doses of PAA, selected cells with increased karyotypic instability, were accompanied by changes in the expression of genes , , , . These changes did not contribute to the progression of MDR, accompanied by the increased sensitivity of these cells to the toxic effects of doxorubicin (DOX). Our results showed that PAA does not increase the oncogenic potential of immortalized cells and confirmed that it can be used for intracellular drug delivery for anticancer therapy.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589997 | PMC |
| http://dx.doi.org/10.3390/cells9102332 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Layer-by-layer assembled film can serve as an enhanced reaction environment for Diels-Alder reaction.
Front Chem
December 2024
Research Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS), Tsukuba, Japan.
Although the Diels-Alder reaction (DA) has garnered significant attention due to its numerous advantages, its long reaction time is a drawback. Herein, we investigated the effects of polarity difference on DA using Layer-by-Layer (LbL) films comprising polycationic polyallylamine hydrochloride and polyanionic poly (styrenesulfonic acid-co-furfuryl methacrylate) [poly (SS--FMA)] as the reaction environment. First, furan composition in poly (SS--FMA) was adjusted to be 19 mol% to achieve good water solubility and layer deposition.
View Article and Find Full Text PDFSupramolecular dextran/polyamine phosphate nanocapsules with smart responsiveness for encapsulation of therapeutics.
J Colloid Interface Sci
December 2024
Department of Chemistry 'Ugo Schiff', University of Florence, via della Lastruccia 3-13, 50019 Sesto Fiorentino (FI), Italy. Electronic address:
The polyallylamine hydrochloride (PAH) polymer is here functionalized with branched and biocompatible polysaccharide dextran (DEX) molecules. Covalent conjugation of DEX to PAH has been achieved through a straightforward reductive amination approach, allowing for a controlled number of DEX chains per PAH polymer (PAH:DEX, n = 0.1, 0.
View Article and Find Full Text PDFRegulating Strain and Suppressing Defect States Through Polymer Ionization and Chemical Chelation for Efficient Inverted Flexible Perovskite Solar Cells.
Small
December 2024
School of Energy Science and Engineering, University of Science and Technology of China, Hefei, 230026, P. R. China.
Flexible perovskite solar cells (FPSCs) have great promise for applications in wearable technology and space photovoltaics. However, the unpredictable crystallization of perovskite on flexible substrates results in significantly lower efficiency and mechanical durability than industry standards. A strategy is investigated employing the polymer electrolyte poly(allylamine hydrochloride) (PAH) to regulate crystallization and passivate defect states in perovskite films on flexible substrates.
View Article and Find Full Text PDFNeuromorphic Computing Primitives Using Polymer-Networked Nanoparticles.
J Phys Chem C Nanomater Interfaces
December 2024
Department of Chemistry, Johns Hopkins University, Baltimore, Maryland 21218, United States.
Nanoparticle networks have potential applications in brain-like computing yet their ability to adopt different states remains unexplored. In this work, we reveal the dynamics of the attachment of polyelectrolytes onto gold nanoparticles (AuNPs), using a bottom-up two-bead-monomer dissipative particle dynamics (TBM-DPD) model to show the heterogeneity of polymer coverage. We found that the use of one polyelectrolyte homopolymer limits the complexity of the possible engineered nanoparticle networks (ENPNs) that can be built.
View Article and Find Full Text PDFSeparation of Magnesium and Lithium Ions Utilizing Layer-by-Layer Polyelectrolyte Modification of Polyacrylonitrile Hollow Fiber Porous Membranes.
Materials (Basel)
November 2024
Institute of Nanoscience and Nanotechnology, National Centre for Scientific Research Demokritos, Patr. Gregoriou E & 27 Neapoleos Street, 15341 Agia Paraskevi, Greece.
This study explores the layer-by-layer (LBL) modification of polyacrylonitrile (PAN) hollow fibers for effective Mg/Li separation. It employs an LBL method of surface modification using polyelectrolytes, specifically aiming to enhance ion selectivity and improve the efficiency of lithium extraction from brines or lithium battery wastes, which is critical for battery recycling and other industrial applications. The modification process involves coating the hydrolyzed PAN fibers with alternating layers of positively charged polyelectrolytes, such as poly(allylamine hydrochloride) (PAH), polyethyleneimine (PEI), or poly(diallyldimethylammonium chloride) (PDADMAC) and negatively charged polyelectrolytes, such as poly(styrene sulfonate) (PSS), to form polyelectrolyte multilayers (PEMs).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!