CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes . Here we review the many design variations of these versatile tools and compare their effectiveness in different eukaryotic systems. Lastly, we highlight several applications of programmable transcriptional activation to interrogate and engineer complex biological processes.
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| http://dx.doi.org/10.1089/crispr.2020.0064 | DOI Listing |
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