AI Article Synopsis
- Folin-Ciocalteu and Fast Blue BB (FBBB) methods are used to measure total phenolics in food, but Folin-Ciocalteu often faces interference issues.
- FBBB shows good performance with fruits and cereals, while its effectiveness in legumes hasn't been tested yet.
- In a study of 24 flour samples, Folin-Ciocalteu had interferences in 75% of cases, while FBBB had some interference only in legumes and nuts; notably, FBBB with Solid Phase Extraction (SPE) was more sensitive.
Article Abstract
Folin-Ciocalteu and the more recent Fast Blue BB (FBBB) reactions are used for the quantification of total phenolics in food matrices. Despite its known interferences, Folin-Ciocalteu is still widely employed, considering Solid Phase Extraction (SPE) as clean-up step only in a few cases. Meanwhile, FBBB has shown no interferences for the determination of total phenolics in fruits and cereals, although its utilization in other popular matrices containing potential interferences, such as legumes, remains unexplored. In this study, the total phenolic content of 24 flours from legumes, cereals, fruits, nuts and plant seeds was evaluated by Folin-Ciocalteu and FBBB, with and without SPE. Folin-Ciocalteu showed interferences for 75% of the flours (attributed to reducing sugars and enediols), whereas FBBB only for legumes and nuts (attributed to the presence of tyrosine), both methods in those matrices requiring SPE. Although both SPE-FBBB and SPE-Folin-Ciocalteu presented excellent reproducibility, SPE-FBBB displayed 1.5 times higher sensitivity.
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| http://dx.doi.org/10.1039/d0fo01857k | DOI Listing |
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