Single-stage partial nitration and anammox (PN/A) process was achieved in a sequencing batch reactor (SBR) using a submerged dynamic membrane (DM) in this study. The reactor was stably operated for 200 days, and the nitrogen removal efficiency (NRE) was sustained at 70.3 ± 7.2% at a nitrogen loading rate (NLR) ranging from 0.1 to 0.3 kgNm day with a hydraulic retention time (HRT) of 24 hr. When the NLR was 0.2 kgN m day , the NRE achieved was high as 80% with a low concentration of dissolved oxygen (DO) of 0.13 mg/L. In addition, the specific activity of anammox bacteria and ammonia-oxidizing bacteria (AOB) reached was 2.72 and 16.80 gN gVSS day , respectively. The DM intercepted the biomass due to the lamellar, intact, dense biofilm self-generated on the surface of the supporting material, which had an effluent turbidity of 10 NTU. The enriched anammox functional bacteria were Candidatus Jettenia (11.06%) and the AOB-like functional bacteria consisted primarily of Nitrosomonas, with a relative abundance of 2.76%, which ensured the PN/A process proceeding. This study provides a novel reactor configuration of the single-stage PN/A process in the view of practical applications. PRACTITIONER POINTS: Single-stage partial nitration and anammox (PN/A) process was achieved using a submerged dynamic membrane (DM) in this study. The reactor was stably operated for 200 days, and the nitrogen removal efficiency was sustained at 70.3 ± 7.2%. The feasibility of the PN/A system with DM is evaluated. The main objective is to provide a control strategy of the DM-SBRs for practical applications.
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http://dx.doi.org/10.1002/wer.1468 | DOI Listing |
Water Res X
May 2025
School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.
Partial Nitritation/Anammox (PN/A) can achieve green, economical, and efficient biological nitrogen removal; however, the PN process contributes significantly to nitrous oxide (NO, the third most important greenhouse gas) emissions. Balancing the stability of PN systems while reducing NO emissions, particularly under varying salinity conditions, is a key challenge in applying PN/A for high-salinity and high-ammonia wastewater treatment. This study explored the long-term effects of salinity on PN performance and NO emissions in PN systems treating high-ammonia wastewater.
View Article and Find Full Text PDFBioresour Technol
May 2025
Faculty of Civil and Environmental Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel.
Nitrous oxide (NO) emissions from partial nitritation- anammox (PN/A) processes for deammonification has become an important issue as the technology has become more widespread, however, NO emissions from PN/A treating hypersaline wastewater has been little reported. Here, NO emissions from completely mixed suspended and attached single-stage PN/A reactors treating hypersaline (4 % salinity; 40 g/kg) wastewater for ammonium removal were first investigated without the addition of organics. Results show that NO emissions in fixed bed and suspended PN/A reactors without organic addition ranged from 0.
View Article and Find Full Text PDFJ Environ Manage
March 2025
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing, 100124, China. Electronic address:
While direct anammox implementation is attractive when treating wastewater, nitrite (NO-N) availability and excess organic matter significantly limit its practical application. This study proposed partial nitrification and endogenous partial denitrification/anammox (PN/A-EPD/A) for the treatment of real municipal wastewater (COD/N ratio: 2.8) within a single-stage reactor under anaerobic/aerobic/anoxic mode.
View Article and Find Full Text PDFJ Hazard Mater
January 2025
East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai 200093, PR China. Electronic address:
Nitrogen conversion via partial nitrification-anammox (PN/A), utilizing nitrite as a key intermediate, is an ideal low-carbon approach for wastewater nitrogen removal. However, the partial nitrification process, which is rate-limited and relies on less common bacteria with slow reaction kinetics, poses challenges for high-throughput PN/A implementation. Herein, we developed a microbial/photocatalysis coupling system using Rhodococcus bacterial and O-doped g-CN (OCN) photocatalysts.
View Article and Find Full Text PDFJ Hazard Mater
March 2025
College of Environmental Science and Engineering, Institute of Carbon Neutrality, State Key Laboratory of Pollution Control and Resources Reuse, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
Anaerobic digestion (AD) reject water serves as a significant reservoir for antibiotic resistance genes (ARGs), underscoring the importance of understanding ARGs dynamics during treatment processes. Partial nitritation /anammox (PN/A) has become an increasingly adopted process, while comprehensive investigation on ARG behavior within this system, especially in full-scale, remains limited. This study explores the distribution of ARGs in a full-scale two-stage PN/A system, with an anaerobic/anoxic/oxic (AAO) system for comparison.
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