Objective: The aim of this study was to investigate the roles of SMURF1 and SMURF2 in progenitor cells from the human knee in late-stage osteoarthritis (OA).
Design: We applied immunohistochemistry, immunocytochemistry, RNAi, lentiviral transfection, and Western blot analysis. We obtained chondrogenic progenitor cells (CPCs) from the articular cartilage and meniscus progenitor cells (MPCs) from the nonvascularized part of the meniscus.
Results: SMURF1 and SMURF2 appeared in both osteoarthritic tissues. CPCs and MPCs exhibited comparable amounts of these proteins, which influence the balance between RUNX2 and SOX9. The overexpression of SMURF1 reduced the levels of RUNX2, SOX9, and TGFBR1. The overexpression of SMURF2 also reduced the levels of RUNX2 and TGFBR1, while SOX9 levels were not affected. The knockdown of SMURF1 had no effect on RUNX2, SOX9, or TGFBR1. The knockdown of SMURF2 enhanced RUNX2 and SOX9 levels in CPCs. The respective protein levels in MPCs were not affected.
Conclusions: This study shows that SMURF1 and SMURF2 are regulatory players for the expression of the major regulator transcription factors RUNX2 and SOX9 in CPCs and MPCs. Our novel findings may help elucidate new treatment strategies for cartilage regeneration.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8721605 | PMC |
| http://dx.doi.org/10.1177/1947603520967069 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of Low-Molecular-Weight Hyaluronate-Based Nanoparticles on the In Vitro Expression of Cartilage Markers.
Int J Mol Sci
November 2024
Food and Drug Department, University of Parma, Parco Area Delle Scienze 27/a, 43124 Parma, Italy.
Hyaluronic acid (HA) is a key component of synovial fluid as it plays a crucial role in joint physiology. Its biological activity is influenced by molecular weight, local concentration, and persistence in joints. High-molecular-weight HA has a consolidated history of clinical use, whereas little is known about the metabolic effect of low-molecular-weight hyaluronate on cartilage differentiation.
View Article and Find Full Text PDFZinc Ameliorates High Pi and Ca-Mediated Osteogenic Differentiation of Mesenchymal Stem Cells.
Nutrients
November 2024
MTA-DE Lendület Vascular Pathophysiology Research Group, Research Centre for Molecular Medicine, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.
Zinc is the second most abundant trace element in the human body, stored mainly in the bones. Zinc is required for bone growth and homeostasis and is also a crucial cofactor for numerous proteins that play key roles in maintaining microstructural integrity and bone remodeling. Bone marrow-derived mesenchymal stem cells (BMSCs) are multipotent progenitors found in the bone marrow stroma and can differentiate along multiple lineage pathways.
View Article and Find Full Text PDFMatrigel-encapsulated articular cartilage derived fibronectin adhesion assay derived chondroprogenitors for enhanced chondrogenic differentiation: An in vitro evaluation.
Tissue Cell
February 2025
Centre for Stem Cell Research, (A unit of InStem, Bengaluru), Christian Medical College, Vellore, India; Department of Physiology, Christian Medical College, Vellore, India. Electronic address:
Purpose: In cartilage research, three-dimensional (3D) culture models are pivotal for assessing chondrogenic differentiation potential. Standard pellet cultures, despite their utility, pose challenges like uneven differentiation and handling difficulties. This study explores the use of Matrigel, an extracellular matrix-based hydrogel, to encapsulate fibronectin adhesion assay-derived chondroprogenitors (FAA-CPs) and evaluate their chondrogenic differentiation potential.
View Article and Find Full Text PDFCulturing 3D chitosan/gelatin/nano-hydroxyapatite and bone-derived scaffolds in a dynamic environment enhances osteochondral reconstruction.
Int J Biol Macromol
December 2024
State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. Electronic address:
Bioreactor can provide a dynamic culture environment for the in vitro construction of osteochondral tissue engineering. They facilitate more efficient exchange of nutrients and provide mechanical and other beneficial stimulation. Previous findings demonstrated that rotary flask (RF) bioreactor, rotary cell culture system (RCCS), or electromagnetic field (EMF) mediated scaffold culture could create a favorable dynamic environment for osteochondral tissue engineering.
View Article and Find Full Text PDFElectrohydrodynamic-Printed Dual-Triphase Microfibrous Scaffolds Reshaping the Lipidomic Profile for Enthesis Healing in a Rat Rotator Cuff Repair Model.
Small
January 2025
Department of Orthopaedics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China.
Article Synopsis
- Rotator cuff injuries cause ongoing pain and limited function, often due to retears and scar tissue at the site where tendons attach to bone (enthesis).
- This study investigates new electrohydrodynamic-printed microfibrous scaffolds (DTSs) made of polycaprolactone mixed with nanoparticles, which show effective stem cell support and enhanced markers for tendon and bone development in lab tests.
- In animal tests, these scaffolds improve the strength of the tendon-bone connection and regulate important phospholipids, suggesting they could lead to better healing outcomes for rotator cuff injuries and pave the way for more tailored treatment options.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!