DNA polymerase mu: An inflexible scaffold for substrate flexibility.

DNA Repair (Amst)

Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, 27709, USA. Electronic address:

Published: September 2020

DNA polymerase μ is a Family X member that participates in repair of DNA double strand breaks (DSBs) by non-homologous end joining. Its role is to fill short gaps arising as intermediates in the process of V(D)J recombination and during processing of accidental double strand breaks. Pol μ is the only known template-dependent polymerase that can repair non-complementary DSBs with unpaired 3´primer termini. Here we review the unique properties of Pol μ that allow it to productively engage such a highly unstable substrate to generate a nick that can be sealed by DNA Ligase IV.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8713560PMC
http://dx.doi.org/10.1016/j.dnarep.2020.102932DOI Listing

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