AI Article Synopsis
- The glnA gene promoter in E. coli, known as glnAp2, is activated by a flexible element that can work in both directions and over varying distances.
- This activating element specifically impacts glnAp2 without affecting the adjacent 82p promoter, which transcribes in the opposite direction.
- The ability of this element to selectively activate glnAp2 is linked to its interaction with different sigma factors that RNA polymerases recognize, suggesting that the right sigma factor is crucial for promoter selection.
Article Abstract
The Escherichia coli glnA gene promoter glnAp2 is activated by an element able to act bidirectionally and at variable distance over the DNA. We demonstrate here that this activating element does not influence another promoter, 82p, adjacent to it, from which a gene is transcribed in opposite direction to glnA. Thus, although it displays a great flexibility, this element can activate selectively. The unresponsive promoter and glnAp2 are recognized by RNA polymerases complexed to two different sigma factors. Therefore, we argue that promoter selection by this element is dependent upon distinguishing the proper sigma factor.
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| http://dx.doi.org/10.1016/0378-1119(87)90497-5 | DOI Listing |
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