AI Article Synopsis
- This study investigates the impact of the EZH2 inhibitor GSK126 on human tongue squamous cell carcinoma cells, focusing on how it affects cell growth and death.
- GSK126 was tested on CAL-27 cancer cells using various assays to measure cell proliferation and apoptosis, revealing that it significantly inhibits cell growth and promotes cell death.
- The results indicate that GSK126 works by down-regulating p-ERK and Bcl-2 while up-regulating Bax and Cleaved caspase-9, suggesting its effects are linked to the suppression of the MEK/ERK pathway and modulation of the Bax/Bcl-2 pathway.
Article Abstract
Objective: This study aims to study the effect of the enhancer of zeste homolog 2 (EZH2) inhibitor GSK126 on the proliferation and apoptosis of human tongue squamous cell carcinoma cells in vitro and explore its related mechanisms in order to obtain insights into the clinical treatment of tongue squamous cell carcinoma.
Methods: Different concentrations of GSK126 were applied to CAL-27 cells of tongue squamous cell carcinoma, and the effects of drugs on cell proliferation were detected through methyl thiazolyl tetrazolium (MTT) assay, colony formation assay, and 5-ethynyl-2'-deoxyuridine (EdU) fluorescence staining. Hoechst33342 fluorescence staining and the JC-1 method were used in observing apoptosis. The expression levels of extracellular regulated protein kinases (ERK), phospho-extracellular regulated protein kinases (p-ERK), Bax, Bcl-2, and Cleaved caspase-9 in Cal-27 cells were detected through Western blot.
Results: GSK126 inhibited CAL-27 cell proliferation and promoted apoptosis. GSK126 down-regulated the expression of p-ERK and Bcl-2 and increased the expression of Bax and Cleaved caspase-9 (P<0.05).
Conclusions: GSK126 can inhibit the proliferation of CAL-27 cells in tongue squamous cell carcinoma and promote its apoptosis, and the related mechanism may be associated with the inhibition of the MEK/ERK signaling pathway and activation of the Bax/Bcl-2 pathway.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573763 | PMC |
| http://dx.doi.org/10.7518/hxkq.2020.05.004 | DOI Listing |
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