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The expression of glycolysis-related proteins in urine significantly increases after running.
Front Physiol
December 2024
Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Beijing, China.
Objective: Glucose metabolism is the main way in which cells obtain energy during exercise and plays an important role in exercise. The purpose of this study was to explore the changes in the expression of glucose metabolism-related proteins in urine after running, and finally applied to the monitoring of running training.
Methods: Urine samples were collected before and after running, and urine proteomics information was collected to explore the expression of proteins in the urine using LC-MS/MS in DDA mode and DIA mode.
Update on NKCC2 regulation in the thick ascending limb (TAL) by membrane trafficking, phosphorylation, and protein-protein interactions.
Front Physiol
December 2024
Department of Internal Medicine, Hypertension and Vascular Research Division, Henry ford hospital, Detroit, MI, United States.
Purpose Of Review: The thick ascending limb (TAL) of loop of Henle is essential for NaCl, calcium and magnesium homeostasis, pH balance and for urine concentration. NKCC2 is the main transporter for NaCl reabsorption in the TAL and its regulation is very complex. There have been recent advancements toward understanding how NKCC2 is regulated by protein trafficking, protein-protein interaction, and phosphorylation/dephosphorylation.
View Article and Find Full Text PDFProteomic Analysis of Biological Fluids.
Methods Mol Biol
December 2024
Department of Environmental Toxicology, The University of California, Davis, Davis, CA, USA.
Biological fluids are proteinaceous liquids or suspensions released through different body orifices or through penetration of the skin. These fluids are the result of multiple tissues and cell types and contain extensive, highly complex, and dynamic protein populations that reflect both the transcriptional program of the originating cells and a record of the individual's health status. Body fluids are readily accessible to clinicians and researchers, and as such proteomic analyses are an important component of clinical studies, fertility studies, oral health studies, and forensic investigations.
View Article and Find Full Text PDFDiagnostic performance of Uromonitor and TERTpm ddPCR urine tests for the non-invasive detection of bladder cancer.
Sci Rep
December 2024
Department of Urology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
Uromonitor and urinary telomerase reverse transcriptase promoter mutation droplet digital PCR (uTERTpm ddPCR) are non-invasive tests designed to detect bladder cancer in urine. We aimed to compare the diagnostic performance of uTERTpm ddPCR, Uromonitor and urine cytology in detecting bladder cancer. Urine samples were collected prospectively from patients diagnosed with primary (n = 74) and recurrent bladder cancer (n = 20) or benign urological conditions (n = 48) prior to surgical resection.
View Article and Find Full Text PDFInvestigating the incremental value of urine sediment reporting in emergency medicine with a Sysmex UN urinalysis system.
Adv Lab Med
December 2024
Section of Clinical Biochemistry and School of Medicine, University of Verona, Verona, Italy.
Objectives: Urinalysis is widely used and is also frequently requested in emergency situations for screening hypovolemia, urinary tract infections, diabetes, ketoacidosis and hematuria. Our aim was to evaluate the impact of reporting urinary sediment in emergency department specimens with the Sysmex UN system.
Methods: We evaluated urinalyses requested by the emergency department over a three-month period and examined red blood cell count interference, compared leukocyte esterase dipsticks to cytofluorimetric leukocyte count and nitrites to cytofluorimetric bacterial count.
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