AI Article Synopsis
- Researchers utilized UHPLC-Q-Orbitrap-MS/MS to analyze and compare the ginsenoside content in white ginseng (WG) versus extruded white ginseng (EWG).
- They identified 45 different saponins, including various forms of ginsenosides, in both types of ginseng.
- Statistical analyses revealed specific ginsenosides that increased in each type, with certain compounds being higher in WG and different ones elevated in EWG, demonstrating the effectiveness of the analytical method used.
Article Abstract
Ultrahigh-performance liquid chromatography Quadrupole-Orbitrap tandem mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) was used to compare the composition of ginsenosides in white ginseng (WG) and extruded white ginseng (EWG). A total of 45 saponins, including original neutral ginsenosides, malonyl-ginsenosides, and chemical transformation of ginsenosides, were successfully identified in both WG and EWG. Multivariate statistical analyses including supervised orthogonal partial least squared discrimination analysis (OPLS-DA) and hierarchical clustering analysis (HCA) were used to analyze components of white ginseng before and after extrusion. As a result, three ginsenosides (malonyl (M)-Rb1, M-Rb2, and M-Rc) were found to be increased in WG, while three ginsenosides (Rb2, Rc, and Rg1) were elevated in EWG. In the OPLS-DA S-plot, the different compositions of ginsenoside that were distinguished between WG and EWG were screened out. Experimental results indicate that the UHPLC-Q-Orbitrap-MS/MS is a useful tool to characterize variations of ginsenosides in WG and EWG.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7563053 | PMC |
| http://dx.doi.org/10.1155/2020/4764219 | DOI Listing |
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