AI Article Synopsis
- CRISPR-Cas9 gene editing has revolutionized research in human cancers by allowing quick examination of how specific mutations affect cell function.
- Droplet-based technology facilitates the analysis of gene edits made by Cas9 in thousands of individual cells, providing valuable data at a micro level.
- The study focuses on Ba/F3 cells modified to carry common mutations in chronic lymphocytic leukemia, enabling precise measurement of mutation combinations and editing effects at the single-cell level.
Article Abstract
CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers. Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands of single cells. Using this technology, we analyze Ba/F3 cells engineered to express single or multiplexed loss-of-function mutations recurrent in chronic lymphocytic leukemia. Our approach reliably quantifies mutational co-occurrences, zygosity status, and the occurrence of Cas9 edits at single-cell resolution.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574538 | PMC |
| http://dx.doi.org/10.1186/s13059-020-02174-1 | DOI Listing |
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