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Prevalence of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus clinical isolates at a university hospital in Northern Cyprus: a pilot study. | LitMetric

Prevalence of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus clinical isolates at a university hospital in Northern Cyprus: a pilot study.

BMC Res Notes

Department of Medical Microbiology and Clinical Microbiology, Faculty of Medicine, Near East University, Near East Boulevard, 99138, Nicosia, Cyprus.

Published: October 2020

AI Article Synopsis

  • This study investigated the presence of Panton-Valentine leukocidin (PVL) in MRSA isolates from patients at a university hospital in Northern Cyprus, addressing a lack of data on this issue in the region.
  • Out of 50 S. aureus strains examined, 3 were identified as methicillin-sensitive, while 68% of the isolates carried the mecA gene indicating methicillin resistance; 27.7% tested positive for the PVL gene.
  • The PVL-positive MRSA was primarily found in inpatients and was most common in samples from deep tracheal aspirates and abscesses/wounds, marking this as the first report of PVL presence among MRSA in a Northern Cyprus

Article Abstract

Objective: Panton-Valentine leukocidin (PVL)-positive methicillin-resistant Staphylococcus aureus (MRSA) is a healthcare problem worldwide. There are no reports on the virulence characteristics of MRSA in Northern Cyprus (NC). This study aimed to determine the presence of pvl among MRSA isolates from patients admitted to a university hospital in NC using molecular methods. Fifty S. aureus strains were included in this study. BD Phoenix automated identification system was used for bacterial identification and antibiotic susceptibility testing. Methicillin resistance was confirmed by disc diffusion assay. Presence of nuc and mecA genes was tested by multiplex PCR. Detection of pvl gene was performed by single-target PCR.

Results: Out of 50 S. aureus isolates identified as MRSA by BD Phoenix system, 3 were susceptible to cefoxitin with disc diffusion assay and were confirmed as methicillin-sensitive S. aureus (MSSA). All isolates (n = 50, 100%) tested positive for the presence nuc gene and 68% (n = 34/50) were mecA positive. pvl was detected in 27.7% (n = 13/47) of the MRSA isolates. Among PVL-positive MRSA isolates, 69.2% (9/13) were inpatients. PVL-MRSA was more common in isolates from deep tracheal aspirate (30.8%, 4/13) and abscess/wound (23.1%, 3/13). This represents the first study of PVL presence among MRSA in hospital setting in NC.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576721PMC
http://dx.doi.org/10.1186/s13104-020-05339-0DOI Listing

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