AI Article Synopsis
- The retinal pigment epithelium (RPE) relies on autophagy to manage high levels of phagocytosis and oxidative stress, with impaired autophagy linked to disorders like dry age-related macular degeneration (AMD) that currently lack effective treatments.
- The study focuses on Cysteinyl leukotriene receptor (CysLTR) 1's role in regulating autophagy in ARPE-19 cells, revealing that CysLTR1 influences autophagic activity and is co-localized with β-tubulin III.
- Results show that inhibiting CysLTR1 with Zafirlukast enhances autophagy and suggests that dysfunction in the leukotriene system could harm RPE functions,
Article Abstract
The retinal pigment epithelium (RPE), which is among the tissues in the body that are exposed to the highest levels of phagocytosis and oxidative stress, is dependent on autophagy function. Impaired autophagy and continuous cellular stress are associated with various disorders, such as dry age-related macular degeneration (AMD), a disease for which effective therapies are lacking. Cysteinyl leukotriene receptor (CysLTR) 1 is a potential modulator of autophagy; thus, the aim of this study was to investigate the role of CysLTR1 in autophagy regulation in the RPE cell line ARPE-19. The polarized ARPE-19 monolayer exhibited expression of CysLTR1, which was colocalized with β-tubulin III. In ARPE-19 cells, autophagic activity was rhythmically regulated and was increased upon CysLTR1 inhibition by Zafirlukast (ZK) treatment. HO affected the proautophagic regulatory effect of ZK treatment depending on whether it was applied simultaneously with or prior to ZK treatment. Furthermore, mRNA levels of genes related to the leukotriene system, autophagy and the unfolded protein response were positively correlated. As CysLTR1 is involved in autophagy regulation under basal and oxidative stress conditions, a dysfunctional leukotriene system could negatively affect RPE functions. Therefore, CysLTR1 is a potential target for new treatment approaches for neurodegenerative disorders, such as AMD.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573618 | PMC |
| http://dx.doi.org/10.1038/s41598-020-74755-w | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cysteine Leukotriene Receptor Antagonist-Montelukast Effects on Diabetic Retinal Microvascular Endothelial Cells Curtail Autophagy.
Invest Ophthalmol Vis Sci
November 2024
Department of Ophthalmology, University of Tennessee Health Science Center, Memphis, Tennessee, United States.
Purpose: Diabetic macular edema (DME) is the primary cause of vision impairment in diabetic retinopathy (DR) patients. A previous study has shown the efficacy of montelukast, a cysteinyl leukotriene receptor (CysLTR)1 antagonist, in a diabetic mouse model. This study aims to understand the CysLTR1 signaling in retinal endothelial cells and the impact of montelukast.
View Article and Find Full Text PDFEffect of Benzyl Alcohol on Main Defense System Components of (Lepidoptera).
Int J Mol Sci
October 2024
Museum and Institute of Zoology, Polish Academy of Sciences, ul. Twarda 51/55, 00-818 Warszawa, Poland.
Article Synopsis
- * This study examined how benzyl alcohol impacts the immune defense systems of wax moths, revealing significant changes in their cuticular lipid composition and immune responses.
- * Findings suggest that benzyl alcohol not only boosts certain protective proteins but also negatively affects insect cells, highlighting its potential as a non-toxic insecticide for vertebrates.
CYSLTR1 antagonism displays potent anti-tumor effects in uveal melanoma.
Exp Eye Res
November 2024
Cancer Research Program, Research Institute of the McGill University Health Centre, Montreal, QC, Canada; The MUHC - McGill University Ocular Pathology & Translational Research Laboratory, Montreal, QC, Canada.
Article Synopsis
- * The study investigates the expression of the CYSLTR1 receptor in 31 human UM samples and shows that it is highly expressed in both primary and metastatic UM cells.
- * Blocking CYSLTR1 with the drug MK571 reduces UM cell growth and metabolic activity while increasing cell death, suggesting that targeting this receptor could be a promising treatment strategy for UM.
Cysteinyl Leukotriene Receptor Antagonism by Montelukast to Treat Visual Deficits.
J Ocul Pharmacol Ther
December 2024
Department of Ophthalmology, Anatomy & Neurobiology, Neuroscience Institute, University of Tennessee Health Science Center, Memphis, Tennessee, USA.
Article Synopsis
- Montelukast is a medicine that helps with asthma and allergies by blocking certain signals in the body that cause inflammation.
- New research shows that Montelukast might also help with other serious problems like heart disease, cancer, and eye diseases.
- The review looks at how Montelukast works in different diseases and checks to see if it can help people with vision problems.
The role of signaling pathways mediated by the GPCRs CysLTR1/2 in melanocyte proliferation and senescence.
Sci Signal
September 2024
Laboratory of Chemical Biology and Signal Transduction, Rockefeller University, New York, NY 10065, USA.
Article Synopsis
- Melanoma caused by sun exposure differs from rarer types of melanocytic tumors, which have fewer mutations and allow researchers to explore specific signaling mechanisms.
- Uveal melanoma and blue nevi are examples of these rarer tumors, often linked to mutations in a particular signaling pathway involving G protein-coupled receptors.
- The study highlights how the same mutations can lead to cell growth in one tissue while hindering growth in another, illustrating how the tissue environment influences cell behavior and balance between growth and aging.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!