Transcriptomic Analysis in the Sea Anemone Nematostella vectensis.

Methods Mol Biol

Université Côte d'Azur, CNRS, INSERM, Institute for Research on Cancer and Aging, Nice (IRCAN), Nice, France.

Published: March 2021

The sea anemone Nematostella vectensis is an emerging research model to study embryonic development and regeneration at the molecular and global transcriptomic level. Transcriptomics analysis is now routinely used to detect differential expression at the genome level. Here we present the latest procedures for isolating high-quality RNA required for next generation sequencing, as well as methods and resources for quantifying transcriptomic data.

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http://dx.doi.org/10.1007/978-1-0716-0974-3_14DOI Listing

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