[Rapid identification of 15 common S. enterica Salmonella serovars by multiplex real-time PCR].

Objective: Multiplex real-time PCR for the identification of 15 Salmonella serovars was developed.

Methods: Through the Salmonella genome comparison, 12 membrane proteins STM4497 gene can be used to identify 15 Salmonella serovars, and these 12 genes were respectively listed as A-L genes. Then primers were designed according to A-L gene conserved sequences, and then multiplex real-time PCR was established assessed with the evaluation of the limit detection, sensitivity, specificity, and repeatability. The 206 Salmonella strains were identified using multiplex real-time PCR with the comparison of the serum slide agglutination assay.

Results: The limit detection of multiplex PCR ranged from 1. 1×10~(-3)-1. 2×10~(-3) ng/μL. The target genes were 100% specificity, and the relative standard deviation was lower than 2. 97%. Compared with the serum slide agglutination assay, Kappa ranged 0. 92-1. 00.

Conclusion: The multiplex real-time PCR can be used to identify 15 Salmonella serovars, which is rapid, accurate and specific.