We report the development of the orthogonal amber-suppressor pair Archaeoglobus fulgidus seryl-tRNA (Af-tRNA)/Methanosarcina mazei seryl-tRNA synthetase (MmSerRS) in Escherichia coli. Furthermore, the crystal structure of MmSerRS was solved at 1.45 Å resolution, which should enable structure-guided engineering of its active site to genetically encode small, polar noncanonical amino acids (ncAAs).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9462667 | PMC |
| http://dx.doi.org/10.1016/j.bmc.2020.115662 | DOI Listing |
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