The protozoan pathogen Trichomonas vaginalis encodes two carbonic anhydrases (CAs, EC 4.2.1.1) belonging to the β-class. One of these enzymes, T. vaginalis carbonic anhydrase 1 (TvaCA1), was recently cloned and characterized by our group, and its X-ray crystal structure reported. No inhibitors of this enzyme were reported up until now. Here we investigated the inhibition of TvaCA1 with inorganic anions and small molecules and observed that thiocyanate, cyanide, selenite, selenocyanate and divanadate are sub-millimolar inhibitors, whereas sulfamide, sulfate, phenylboronic acid and phenylarsonic acid are micromolar inhibitors. Finding effective TvaCA1 inhibitors may be useful for developing new antiprotozoan drugs.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jinorgbio.2020.111274 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Construction and biological function of gene knockout strain.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
August 2024
Department of Parasitology, School of Basic Medical Sciences, Central South University, Changsha 410013.
Objectives: Toxoplasmosis is a zoonotic parasitic disease caused by (), which can lead to complications such as encephalitis and ocular toxoplasmosis. The disease becomes more severe when the host's immune system is compromised. Rhoptry proteins are major virulence factors that enable to invade host cells.
View Article and Find Full Text PDFResearch note: The critical role of the interaction between Eimeria tenella invasion protein RON2 and host receptor annexin A2 in mediating parasite invasion.
Poult Sci
December 2024
Guangdong Province Key Laboratory of Livestock Disease Prevention, Key Laboratory of Avian Infuenza and Other Major Poultry Diseases Prevention and Control, Ministry of Agriculture and Rural Afairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China. Electronic address:
Avian coccidiosis, caused by protozoan parasites of the genus Eimeria, is a globally prevalent and highly pathogenic disease that poses a serious threat to the poultry industry, resulting in significant economic losses. However, the mechanism by which Eimeria species invade host cells remains unclear. Previous studies have identified rhoptry neck protein 2 (RON2) from Eimeria tenella as a critical factor in host cell invasion, but a comprehensive understanding of the role of EtRON2 in host cell invasion and its relationship with E.
View Article and Find Full Text PDFPlasmodium falciparum histidine-rich protein 2 exhibits cell penetration and cytotoxicity with autophagy dysfunction.
Biosci Biotechnol Biochem
January 2025
Department of Bioresource Science, Faculty of Agriculture.
Plasmodium falciparum is a major cause of severe malaria. This protozoan infects human red blood cells and secretes large quantities of histidine-rich protein 2 (PfHRP2) into the bloodstream, making it a well-known diagnostic marker. Here, however, we identified PfHRP2 as a pathogenic factor produced by P.
View Article and Find Full Text PDFCascading expression of ApiAP2 transcription factors controls daughter cell assembly in Toxoplasma gondii.
PLoS Pathog
December 2024
University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, Lille, France.
Pathogenesis of Toxoplasma gondii in the intermediate host is based on the tachyzoite ability to divide rapidly to produce significant amount of daughter cells in a reduce time frame. The regulation of the cell-cycle specific expression program is therefore key to their proliferation. Transcriptional regulation has a crucial role in establishing this expression program and transcription factors regulates many aspects of tachyzoite cell cycle.
View Article and Find Full Text PDFSurvey of parasitic fauna data from wild animals through coproparasitological diagnosis in Southern Brazil.
BMC Vet Res
January 2025
Laboratório de Epidemiologia Veterinária, Departamento de Veterinária Preventiva, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brasil.
Background: The proximity between people and their domestic animals with wild animal populations can result in the spread of diseases with a significant impact on public health. Infection by parasites in wildlife is considered an important bioindicator of the current state of ecosystems, and studying the epidemiology of these infections is essential for a better understanding of natural foci. However, research on parasites in southern Brazil, especially in Rio Grande do Sul (RS), is considered incipient.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!